Table s4 Tissues



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TABLE S4

Tissues

Bone:


Derivation: Surgical Tissue

Sources: Normal bone, from hip replacement for osteonecrosis of head of femur in 38 y/o white male; Normal bone, from amputation of forearm for osteosarcoma of radius in 29 y/o white male; Normal fibula from 66 yr old while female

Microarray facility: GeneLogic
Brain-

Derivation: Postmortem tissue

Sources: Occipital cortex from 49 y/o male, Frontal cortex from 69 y/o male, Occipital cortex from 56 y/o male

Supplier: Cooperative Human Tissue Network

Microarray facility: Genentech
Fetal brain:

Derivation: Postmortem tissue

Sources: Fetal brain tissue, 15 weeks, 16 weeks, 20 weeks gestation

Supplier: Advanced BioScience Resource

Microarray facility: Genentech
Synovium-

Derivation: Surgical tissue

Sources: Synovial membranes from patients with osteosrthritis (n=2) or rheumatoid arthritis (n=1), dissected free of fat, cartilage, and bone

Microarray facility: Genentech




Isolated cells or primary cultures

Astrocytes-

Derivation: Primary culture

Source: Clonetics® Astrocyte Cell System

Culture media: Astrocyte medium from Clonetics with SingleQuots®

Microarray facility: Genentech


Dendritic cells-

Derivation: Primary culture

Source: Differentiated in vitro from CD14+ monocytes

Culture conditions: CD14+ monocytes treated with IL4 (17 ng/ml) and GMCSF (67 ng/ml) for 5 days At 2.5 X 106 cells/ml

Microarray facility: Genentech


Endothelial cells:

Derivation: Primary culture

Sources: Aortic endothelial cell or Umbilical vein endothelial cell

Cell confluence at harvest: 95

Freezing medium: 10% DMSO

Culture gases: 5% CO2

RNA extraction: TRIzol

Growth medium: Cascade M-200-500 + supplements

Passage: 3

Culture temperature: 37 C

Culture vessel: T-175 flask
Hematopoetic stem cells:

Derivation: Isolated cells

Sources: CD34+ cells (immunophenotype for CD34: 97.0%- 99.1%) from normal donors, 30 y/o Hispanic female; 23 y/o white female; 23 y/o white male and, 22 y/o white female, 20 y/o Asian male

Microarray facility: GeneLogic


Smooth muscle-

Derivation: Primary culture

Source: Tunica media of aorta, coronary artery, and pulmonary artery

Cell confluence at harvest: 95

Freezing medium: 10% DMSO

Culture gases: 5% CO2

RNA extraction: TRIzol

Growth medium: Cascade M-200-500 + supplements

Passage: 3

Culture temperature: 37 C

Culture vessel: T-75 flask

Microarray facility: GeneLogic




Cell lines

Jurkat-


Derivation: Cell line

Source: Jurkat acute T-cell leukemia cell line

Culture mode: Suspension

Culture gases: 5% CO2

Harvest method: Centrifugation

Growth medium: RPMI 1640 Media + supplements

Culture temperature: 37 C

Culture vessel: Flask

Microarray facility: GeneLogic
Neural stem cell line 1-

Derivation: Cell line

Source: HH580M human fetal hippocampal stem cell

Culture mode: Adherent

Culture gases: 5% CO2

Harvest method: TRIzol

Growth medium: Neurobasal Medium + supplements

Passage: 9

Culture temperature: 37 C

Culture vessel: 6-well plate

Microarray facility: GeneLogic
Neural stem cell line 1*-

Derivation: Cell line

Source: Treated HH580M human hippocampal stem cell

Culture mode: Adherent

Culture gases: 5% CO2

Harvest method: TRIzol

Growth medium: Neurobasal Medium + supplements

Passage: 9

Culture temperature: 37 C

Culture vessel: 6-well plate

Comments: Cell line cultured minus BDNF to select for astrocytes

Microarray facility: GeneLogic


Neural stem cell line 2-

Derivation: Cell line

Source: HK532M human cerebral cortex stem cell

Culture mode: Adherent

Culture gases: 5% CO2

Harvest method: TRIzol

Growth medium: Neurobasal Medium + supplements

Passage: 9

Culture temperature: 37 C

Culture vessel: 6-well plate

Microarray facility: GeneLogic
Neural stem cell line 2*-

Derivation: Cell line

Source: Treated HK532M human hippocampal stem cell

Culture mode: Adherent

Culture gases: 5% CO2

Harvest method: TRIzol

Growth medium: Neurobasal Medium + supplements

Passage: 11

Culture temperature: 37 C

Culture vessel: 6-well plate



Comments: Cell line cultured minus BDNF to select for astrocytes

Microarray facility: GeneLogic
Directory: cms -> attachment
attachment -> Combined antegrade-retrograde recanalization of a completely obstructed esophagus. All authors have no conflicts of interest. We will be discussing a 76-year-old woman with htn
attachment -> Ocular manifestations of the Johanson-Blizzard syndrome
attachment -> Supplementary Material, File 2
attachment -> Supplemental Table S4: Genes Tested in Detail
attachment -> Radiofrequency ablation is a treatment option for early stages of verrucous esophageal carcinoma
attachment -> Supplementary Table gene expression of tyrosine kinases and phosphatases during disease
attachment -> Successful removal of an esophageal submucosal tumor by submucosal tunneling endoscopic resection technique. This is a 32-year-old male with a history of dysphagia
attachment -> Supplemental data
attachment -> Supplementary Table 1: Enriched genes in early mitosis
attachment -> Supplemental material expedition trials mri data acquisition


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