a The position of the strain on the Cas9 tree is given. Color shading corresponds to the color branch of the tree. Compare with Figure 4.
b Compare the sequences with Supplementary Table S4. The predicted or previously validated repeat sequence followed by the hexanucleotide loop, represented as a string of 6 N, in red, and the tracrRNA sequence is shown. Below, the predicted secondary structure of the RNA is represented with a dot and bracket notation. The first stem-loop downstream of the anti-repeat region, when not corresponding to a putative transcriptional terminator is underlined. Note, that in type II-B loci the stem-loop found in S. wadsworthensis and P. excrementihominis is formed within the putative anti-repeat.