Suitability of Various Specimens for Anaerobic Culture Site Acceptable specimens Unacceptable specimens



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Suitability of Various Specimens for Anaerobic Culture
Site Acceptable specimens Unacceptable specimens

Head and Neck

*Abscess aspirate obtained by needle and syringe after surface decontamination.

*Biopsy material surgically obtained.

*Anaerobic swab surgically obtained when aspirate not feasible.


*Throat or nasopharyngeal swabs

*Gingival swabs

*Superficial material collected on swabs


Lungs

*Transtracheal aspirate

*Material from percutaneous lung puncture.

*Bronchoscopic specimen obtained by protected brush.

*Thoracotomy specimen.

*Anaerobic swab surgically obtained.


*Expectorated sputum

*Induced sputum

*Endotracheal aspirate

*Bronchoscopic specimens not specially collected



Central Nervous System

*Abscess aspirate obtained by needle and syringe.

*Biopsy material surgically obtained.

*Anaerobic swabs surgically obtained.


*Aerobic swabs

Abdomen

*Peritoneal fluid obtained by needle and syringe.

*Abscess aspirate obtained by needle and syringe.

*Bile

*Biopsy material surgically obtained.



*Anaerobic swab surgically obtained.

*Aerobic swabs

Urinary Tract

*Suprapubic aspirate

*Voided urine

*Catheterized urine



Genital Tract

*Culdoscopy specimens

*Endometrial aspirate obtained by suction or protected collector

*Abscess aspirate obtained by needle and syringe.

*Biopsy material surgically obtained (Placenta, fallopian tube)

*Anaerobic swabs surgically obtained

* IUD for Actinomyces species or Eubacterium nodatum.



*Vaginal, vulvar or cervical secretion swabs

* Lochia secretions

* Prostatic or seminal fluid

*Perineal swab

* Urethral secretions


Bone and Joint

*Aspirate obtained by needle and syringe

*Biopsy material surgically obtained

*Anaerobic swab surgically obtained

*Bone marrow or Blood culture



*Superficial material collected with swabs.

Soft Tissue

*Aspirate obtained by needle and syringe.

*Biopsy material surgically obtained.

*Aspirate from sinus tract obtained by needle and small plastic catheter.

*Deep aspirate of open-wound margin obtained through decontaminated skin.



*Deep aspirate of surface ulcer obtained through decontaminated skin.

*Superficial material collected from skin surface or edges of wound


Large bowel

Only for toxin assay for suspected Clostridium difficile

Stool or Rectal swab



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