This is an SOP template and is not complete until: 1) lab specific information is entered into the box below 2) lab specific protocol/procedure is added to the protocol/procedure section and
3) SOP has been signed and dated by the PI and relevant lab personnel.
Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
Date SOP was written:
Date SOP was approved by PI/lab supervisor:
Internal Lab Safety Coordinator/Lab Manager:
(Name and Phone Number)
Location(s) covered by this SOP:
Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class
Sodium hypochlorite commonly known as bleach is most frequently used as a disinfecting agent. It is a broad-spectrum disinfectant that is an effective disinfectant for enveloped viruses (e.g. HIV, HBV, HSV), vegetative bacteria (e.g. Pseudomonas, Staphylococcus, and Salmonella), fungi (e.g. Candida), mycobacterium (e.g. M. tuberculosis and M. bovis), and nonenveloped viruses (e.g. Adenovirus and Parvovirus). A 1:10 dilution of household bleach (5-6%) is sufficient to disinfect liquid biological waste listed above given a contact time of 20 minutes. Bleach solutions will degrade overtime to solutions of salt and water. Shelf life of standard Clorox bleach is between 6 months and 1 year. A 1:10 dilution of household bleach only has a shelf life of 24 hours and should be disposed of after this time.
Physical & Chemical Properties/Definition of Chemical Group
Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency – Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Needle stick/punctureexposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
Lab coats must be decontaminated before they are removed for laundering. This may be accomplished by washing the affected area in small container of soap and water. Dispose of the soap and water as hazardous waste.
Laboratory work surfaces and equipment shall be decontaminated at the conclusion of each procedure and at the end of each day. Use a soapy, wet paper towel to clean the affected areas and dispose of the paper towel as hazardous waste.
General hazardous waste disposal guidelines:
Affix an on-line hazardous waste tag on all waste containers using the WASTe Online Tag Program https://ehs.ucop.edu/waste as soon as the first drop of waste is added to the container
Store hazardous waste in closed containers, in secondary containment and in a designated location
Double-bag dry waste using transparent bags https://www.ehs.ucla.edu/hazwaste/management/containers
Waste must be under the control of the person generating & disposing of it
Dispose of Waste
Dispose of regularly generated chemical waste within 90 days
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure (Add lab specific Protocol/Procedure here)
Low concentration solutions 5-6% available chlorine (household bleach)
0-4 L of undiluted stock solution
Example Procedures Sodium hypochlorite (bleach) is most commonly used to disinfect certain biohazardous waste bacteria, viruses, and fungi. Below are protocols for disinfecting both liquid and solid waste.
1:10 bleach solution for disinfection of solid biological waste
In a chemical fume hood transfer 50 ml sodium hypochlorite (5-6% available chlorine) to a bottle containing 450 ml deionized water.
For solid biological waste that is come in contact with bacteria, viruses, and fungi, expose surfaces that have directly come into contact with these substances to a diluted bleach solution. Expose contaminated surfaces to dilute bleach solution for 20 minutes. Collect liquid and solid waste and dispose of as hazardous chemical waste.
For liquid biological waste that contains bacteria, viruses, and fungi, add a household bleach solution (5-6%) to liquid waste at a 1:10 ratio. Leave in contact for 20 minutes. Collect liquid waste and dispose of as hazardous chemical waste. As an example, 10 ml of biological liquid waste would require the addition of 1 ml household bleach solution (5-6%).
NOTE Any deviation from this SOP requires approval from PI.
Documentation of Training (signature of all users is required)
Prior to conducting any work with sodium hypochlorite, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.
The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer.
The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year.