Standard Operating Procedure Sodium Hypochlorite

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Standard Operating Procedure

Sodium Hypochlorite

This is an SOP template and is not complete until: 1) lab specific information is entered into the box below 2) lab specific protocol/procedure is added to the protocol/procedure section and
3) SOP has been signed and dated by the PI and relevant lab personnel.

Print a copy and insert into your

Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.


Date SOP was written:

Date SOP was approved by PI/lab supervisor:

Principal Investigator:

Internal Lab Safety Coordinator/Lab Manager:

Lab Phone:

Office Phone:

Emergency Contact:

(Name and Phone Number)

Location(s) covered by this SOP:

(Building/Room Number)

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class


Sodium hypochlorite commonly known as bleach is most frequently used as a disinfecting agent. It is a broad-spectrum disinfectant that is an effective disinfectant for enveloped viruses (e.g. HIV, HBV, HSV), vegetative bacteria (e.g. Pseudomonas, Staphylococcus, and Salmonella), fungi (e.g. Candida), mycobacterium (e.g. M. tuberculosis and M. bovis), and nonenveloped viruses (e.g. Adenovirus and Parvovirus). A 1:10 dilution of household bleach (5-6%) is sufficient to disinfect liquid biological waste listed above given a contact time of 20 minutes. Bleach solutions will degrade overtime to solutions of salt and water. Shelf life of standard Clorox bleach is between 6 months and 1 year. A 1:10 dilution of household bleach only has a shelf life of 24 hours and should be disposed of after this time.

Physical & Chemical Properties/Definition of Chemical Group

Class: Health Hazard

CAS #: 7681-52-9
Formula: ClNaO
Form: liquid
Color: colorless
Potential Hazards/Toxicity

Emergency Overview

OSHA Hazards


Target Organs

Skin, eyes

GHS Classification

Skin corrosion (Category 1B)

Serious eye damage (Category 1)

Acute aquatic toxicity (Category 1)

GHS Label elements

Signal word: Danger

Hazard statement(s)

H314 Causes severe skin burns and eye damage.

H400 Very toxic to aquatic life.
Precautionary statement(s)

P273 Avoid release to the environment.

P280 Wear protective gloves/ protective clothing/ eye protection/ face protection.

P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

P310 Immediately call a POISON CENTER or doctor/ physician.
Personal Protective Equipment (PPE)

Respiratory Protection

A ½ or full face respirator equipped with appropriate cartridges should be used any time there is the potential for exposure to vapor and/or dust and a fume hood cannot be used

Respirators should be used only under any of the following circumstances:

  • As a last line of defense (i.e., after engineering and administrative controls have been exhausted).

  • When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will be exceeded.

  • Regulations require the use of a respirator.

  • An employer requires the use of a respirator.

  • There is potential for harmful exposure due to an atmospheric contaminant (in the absence of PEL)

  • As PPE in the event of a chemical spill clean-up process

Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S. This is a regulatory requirement. (

Hand Protection
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible

Refer to glove selection chart from the links below:




Eye Protection

ANSI approved safety glasses or goggles are recommended.

Skin and Body Protection

Lab coat, long pants, and closed-toe shoes are required.

Hygiene Measures

After working with chemical, immediately remove gloves, wash hands and arms with soap and water.

Engineering Controls

Work with chemical in a certified fume hood.

First Aid Procedures

If inhaled

Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician

In case of skin contact

Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention immediately

In case of eye contact

Immediately flush eyes with copious amounts of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention immediately

If swallowed

Wash out mouth with water provided person is conscious. Never give anything by mouth to an unconscious person. Call a physician.

Special Handling and Storage Requirements

Precautions for safe handling

Avoid inhalation of vapour or mist.

Conditions for safe storage

Keep container tightly closed in a dry and well-ventilated place.

Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Never allow product to get in contact with water during storage. Do not store near acids.

Recommended storage temperature: 2-8 ° C.
Spill and Accident Procedure

Chemical Spill Dial 911 and x59797

Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Medical Emergency Dial 911 or x52111

Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency – Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.

Decontamination/Waste Disposal Procedure

Lab coats must be decontaminated before they are removed for laundering. This may be accomplished by washing the affected area in small container of soap and water. Dispose of the soap and water as hazardous waste.

Laboratory work surfaces and equipment shall be decontaminated at the conclusion of each procedure and at the end of each day. Use a soapy, wet paper towel to clean the affected areas and dispose of the paper towel as hazardous waste.

General hazardous waste disposal guidelines:

Label Waste

  • Affix an on-line hazardous waste tag on all waste containers using the WASTe Online Tag Program as soon as the first drop of waste is added to the container

Store Waste

  • Store hazardous waste in closed containers, in secondary containment and in a designated location

  • Double-bag dry waste using transparent bags

  • Waste must be under the control of the person generating & disposing of it

Dispose of Waste

  • Dispose of regularly generated chemical waste within 90 days

  • Call EH&S at x61887 for questions

  • Empty Containers

  • Dispose as hazardous waste if it once held extremely hazardous waste (irrespective of the container size)

  • Consult waste pick-up schedule

Prepare for transport to pick-up location

  • Check on-line waste tag

  • Write date of pick-up on the waste tag

  • Use secondary containment

Safety Data Sheet (SDS) Location
Online SDS can be accessed at

Protocol/Procedure (Add lab specific Protocol/Procedure here)

Low concentration solutions 5-6% available chlorine (household bleach)

0-4 L of undiluted stock solution


Example Procedures
Sodium hypochlorite (bleach) is most commonly used to disinfect certain biohazardous waste bacteria, viruses, and fungi. Below are protocols for disinfecting both liquid and solid waste.
1:10 bleach solution for disinfection of solid biological waste

In a chemical fume hood transfer 50 ml sodium hypochlorite (5-6% available chlorine) to a bottle containing 450 ml deionized water.

For solid biological waste that is come in contact with bacteria, viruses, and fungi, expose surfaces that have directly come into contact with these substances to a diluted bleach solution. Expose contaminated surfaces to dilute bleach solution for 20 minutes. Collect liquid and solid waste and dispose of as hazardous chemical waste.
For liquid biological waste that contains bacteria, viruses, and fungi, add a household bleach solution (5-6%) to liquid waste at a 1:10 ratio. Leave in contact for 20 minutes. Collect liquid waste and dispose of as hazardous chemical waste. As an example, 10 ml of biological liquid waste would require the addition of 1 ml household bleach solution (5-6%).
Any deviation from this SOP requires approval from PI.

Documentation of Training (signature of all users is required)

  • Prior to conducting any work with sodium hypochlorite, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.

  • The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer.

  • The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year.

Principal Investigator or Lab Supervisor SOP Approval

Print name_________________________Signature___________________________

Approval Date:

I have read and understand the content of this SOP:




Sodium Hypochlorite Page of Date: 9/16/2015

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