Open the front door/bottom drawer of the Gel-Doc system and clean the
glass plate with 70%ethanol and Kimwipes. Wait until all of the ethanol has evaporated and place gel inside. Make sure that you only touch the Gel-doc with Nitrile gloves as Ethidium Bromide contamination is likely.
Ethidium Bromide – very toxic by inhalation and skin contact
UV illumination is shut down as soon as doors are open
3. At the Gel-doc, turn on the UV transillumination light (from the panel found on the right-hand side of the Gel-Doc) and then activate the “Live/Focus” on the XRS window on the computer.
4. If necessary (i.e. if you have white stripes on image) adjust the lens manually by moving the black lever on top of the Gel-Doc. You can fine tune the image by using the three sets of buttons beneath the “Live/Focus” button which will allow you to focus, zoom and sharpen.
5. Once the image quality is satisfactory, press the “Freeze” button in the XRS window, then select “Automatic aquire”. The gel image will be displayed in a separate window. Once this window has opened, select “File” from the top bar menu and “Export as JPEG”. You can now save your image as a jpeg file onto the desktop, to be exported to your network folder.