Safe work procedure (swp)



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SAFE WORK PROCEDURE (SWP)





SWP Title: Gel Doc System – to photograph a gel


 

SWP Author

Name: Dominique Dolgener

Date: 24/03/2008

OHS Approver

Name: Fiona Richards

Date: 28/03/2008

Supervisor/manager Approver

Name: Pavel Bitter

Date: 28/03/08

Procedure (in steps):

Identify Possible Hazards:

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Controls:

Ref.

  1. Open the front door/bottom drawer of the Gel-Doc system and clean the
    glass plate with 70%ethanol and Kimwipes. Wait until all of the ethanol has evaporated and place gel inside. Make sure that you only touch the Gel-doc with Nitrile gloves as Ethidium Bromide contamination is likely.

  • Ethidium Bromide – very toxic by inhalation and skin contact

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  1. Always wear gloves when working on the computer. Open the Gel-doc

Software (Quantity One). Select “File” from the top bar menu and then

“ChemiDoc XRS”. In the XRS window, press Select and choose “UV



Transillumination” from the drop-down menu.

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    • UV illumination is shut down as soon as doors are open

3. At the Gel-doc, turn on the UV transillumination light (from the panel found on the right-hand side of the Gel-Doc) and then activate the “Live/Focus” on the XRS window on the computer.




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4. If necessary (i.e. if you have white stripes on image) adjust the lens manually by moving the black lever on top of the Gel-Doc. You can fine tune the image by using the three sets of buttons beneath the “Live/Focus” button which will allow you to focus, zoom and sharpen.




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5. Once the image quality is satisfactory, press the “Freeze” button in the XRS window, then select “Automatic aquire”. The gel image will be displayed in a separate window. Once this window has opened, select “File” from the top bar menu and “Export as JPEG”. You can now save your image as a jpeg file onto the desktop, to be exported to your network folder.




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OHS 13 – 150507



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