HISTOLOGICAL EVALUATION OF PULP TO PROPOLIS IN RABBIT TEETH: AN IN VIVO STUDY.
BRIEF RESUME OF THE INTENDED WORK.
6.1 NEED FOR THE STUDY:
Vital pulp therapy is the placement of a biocompatible agent on the remaining pulp tissue that has been inadvertently exposed during cavity preparation or traumatic injury.
Amongst the various materials used for pulp therapy, calcium hydroxide has been the standard by which all others were judged and it is generally accepted as the agent of choice.1
The recent trend is to use natural materials in dental practice. One such natural material is propolis.It is a sticky filler substance with the aroma of poplar honey and vanilla that is collected by bees from the buds and barks of trees and plants .2 It has been proven to have anti-bacterial, anti-viral, anti-inflammatory and anti-fungal effects. But literature on its effects on the pulp is sparse.
This initiated the present study to evaluate the histological reaction of pulp to propolis.
6.2 REVIEW OF LITERATURE:
Calcium hydroxide is a multipurpose agent, and there has been an increasing number of indications for its use .Some of its indications include direct and indirect pulp capping,apexogenesis,apexification,treatment of; root resorption,iatrogenic root perforations, root fractures, replanted teeth and interappointment intracanal dressing. This paper reviewed the properties ,mechanism of antibacterial activity, and various indications for the use of calcium hydroxide.1
Propolis has medical and dental uses especially anaesthetic, antibacterial, antibiotic, anti-inflammatory, antifungal, antiviral and anti-oxidant properties. Propolis is used in dentistry for the repair of surgical wounds, treatment of root canal and periodontitis, application of propolis to dental sockets and skin wounds, direct and indirect dental pulp capping, effect on dentinal hypersensitivity.2
The study wasconducted to assess the response of rat dental pulp to direct pulp capping with propolis flavonoids and non-flavonoids. It was concludedthat direct pulp capping with propolis flavonoidsin rats may delay dental pulp inflammation and stimulate reparative dentin.3
The study was conducted to investigate the effects of Caffeic acid phenethyl ester (CAPE) on wound healing capacity and repair of the pulp dentin complex on an experimental model. Caffeic acid phenethyl ester (CAPE) is an active component of propolis.It was concluded that capping material containing CAPE could not enhance the repair of the dentin-pulp complex, and healing of the dentin-pulp complex was characterized by scar tissue formation, rather than dentin bridge formation.4
6.3 OBJECTIVES OF THE STUDY:
1) To evaluate histologically the reaction of pulp to propolis.
2) To compare the reaction of pulp between propolis and calcium hydroxide.
MATERIALS AND METHOD.
7.1 SOURCE OF DATA:
1) Rabbits to be procured from a certified animal house and the procedure will be carried out under the supervision of a veterinary doctor at the Government Animal Husbandry and Veterinary Hospital, Virajpet, Kodagu.
2) Histology department CIDS, Virajpet.
7.2 METHOD OF COLLECTION OF DATA:
The 30 rabbit molar teeth will be selected.
Control group: Group 1 -Six rabbit molars.
Experimental group: Group 2 -Twelve rabbit molars for propolis.
Group 3 - Twelve rabbit molars for calcium hydroxide.
The procedure will be carried out in 30 rabbit molar teeth.
The rabbits will be anesthetised with the help of a veterinary surgeon .Direct pulp capping procedure will be carried out in the experimental group with propolis as the medicament in group II and with calcium hydroxide in group III. The teeth from group I consisting of six molars will not receive any treatment as it will serve as a control.
The teeth from the control group will be extracted. In the experimental group out of the twenty four molars, six molars from group II and group III will be extracted after one day and the remaining six molars from both the groups will be extracted after one week and subjected to histological evaluation.
DOES THE STUDY REQUIRE ANY INVESTIGATIONS OR INTERVENTIONS TO BE CONDUCTED ON PATIENTS OR OTHER HUMANS OR ANIMALS? IF SO, PLEASE DESCRIBE BRIEFLY.
Yes, pulp therapy procedure will be carried out in the molar teeth of rabbits.
Has ethical clearance been obtained from your institution regarding 7.3?
LIST OF REFERENCES:
1) Alizera Farhad and Zahed Mohammedi.Calcium hydroxide: A Review. International Dental Journal 2005;55:293-301.
2) Khalid Almas, Afaf Dahlan, Ameira Mahmoud.Propolis as a natural remedy: An update .Saudi Dental Journal 2001; 13(1):46-50.
3) Ardo Sabir, Charles R.Tabbu, Purwanto Agustiono, Wihaskoro Sosroseno. Histological analysis of rat dental pulp tissue capped with propolis. Oral Sci 2005; 47(3):135-8.
4) Djurica G, Danilovic Vesna and Krsljak Elena. The effect of caffeic acid phenethyl ester on healing capacity and repair of the dentin –pulp complex: in vivo study.Acta Veterinaria (Beograd) 2004; 58(1):99-108.