A, B) Expression of SUZ12 (A) and EED (B) gene as assessed by RTqPCR (respect to TBP) in normal and tumor samples. p values were obtained by the Mann Whitney t-test, mean and SEM are denoted in red.
Supp. Figure S6 Expression of E2F1, 2 and 3b genes in human NMIBC.
A-B) Expression of E2F1, E2F2 and E2F3b as assessed by RTqPCR (respect to TBP) in normal and tumor samples (A) and in recurrent and non-recurrent tumors (B). p Values were obtained by the Mann Whitney t-test; mean and SEM are shown.
C, E, G). Expression of EZH2gene as a function of E2F1 (C), E2F2 (E) or E2F3B (G) gene expression as assessed by RTqPCR in NMIBC samples. Spearman correlation coefficients (r) and the corresponding p values are provided.
D, F, H) Kaplan-Meier distribution of recurrence in our patient series according the median of the E2F1 (D), E2F2 (F)or E2F3B (H) gene expression. p values were obtained by the Log rank test. n denotes the number of samples scored of each group.
Supp Table S1 Sequence of the oligonucleotides used in RTqPCR experiments HUMAN EZH2 RTsp: 5’-CCTGTCGACATGTTTTGGTC-3’
F 5’- CGGTGTCAAACGCCAATAAA-3’
R 5’- CCGCTCCACTCCACATTCTC-3’
EED RTsp: 5’-TGCTTCCTACAGTTGCAAACAC-3’
F 5’- GAACGCCCTGATACACCTACAAA-3’
R 5´- CATTTTCCCTTCCCCCAACT-3’
SUZ12 RTsp: 5’-CTCTTCTTCCTGGACGAGTCA-3’
F 5’- TCCGATAAGGCAAGTTCCTACAG-3’
R 5’- GAAGTTTCCAGGTTTTGTTTGATTG-3’
E2F1 RTsp: 5’- GTATAAATTAAATGTTTCCA-3’