In vivo disruption of an Rb-E2f-ezh2 signaling loop causes bladder cancer Mirentxu Santos1, 2


Supp Fig 2. Determination of effective elimination of pRb and p130 in bladder tumors



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Supp Fig 2. Determination of effective elimination of pRb and p130 in bladder tumors.

A) Immunoblot of pRb and p130 of control bladder and isolated tumors from RbF/F;p130F/F;p107-/- mice upon AdCre infection. Actin was used to normalize loading.

B, B’) RTqPCR analyses of the expression of Rb1 (B) and Rbl2 (B’) genes in control bladder and isolated tumors from RbF/F;p130F/F;p107-/- mice upon AdCre infection.

C, D) Representative examples of the immunohistochemistry analysis of pRb (C) and p130 (D) expression in mouse bladder tumor sections from RbF/F;p130F/F;p107-/- mice upon AdCre infection.. Note the absence of staining in tumor areas (denoted by T) compared to stroma (denoted by S). Dashed lines mark the tumor-stroma boundaries. Bar=150m





Supp Fig 3 Expression of E2F1, E2F2 and E2F3a genes in mouse bladder tumors.

Expression of E2f1, E2f2 and E2f3a genes in control (uninfected bladder samples) and mouse bladder tumors were monitored by RTqPCR (respect to Tbp). p values were obtained by the Mann Whitney t-test, mean and SEM are shown.






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