Standard Operating Procedure Cacodylic acid



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Standard Operating Procedure

Cacodylic acid

This is an SOP template and is not complete until: 1) lab specific information is entered into the box below 2) lab specific protocol/procedure is added to the protocol/procedure section and
3) SOP has been signed and dated by the PI and relevant lab personnel.

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Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.

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Date SOP was written:

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Principal Investigator:

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Type of SOP: ☐ Process ☒Hazardous Chemical ☒ Hazardous Class

Purpose

Cacodylic acid is used as a buffering agent in chromatography/crystallization, and can be used in conjunction with fixatives for preserving cellular structure. This is a highly toxic compound, so care must be taken when using it. It is used at a final concentration of 10 mM to 100 mM in chromatograph/crystallization buffers as well as fixation solutions.



Advantages:

1.     Easy to prepare.

2.     Stable during storage for long periods of time.

3.     Does not support growth of microorganisms.

4.     Precipitates usually do not occur.  Precipitates do not occur at low concentrations of calcium.

Disadvantages:

1.     Toxic. Contains arsenic.

2.     Unpleasant smell.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 75-60-5

Class: Carcinogen (Prop-65 listed)

Molecular Formula: C2H7AsO2

Form (physical state): Crystalline

Color: White

Melting point/range: 195 - 196 °C (383 - 385 °F)

Synonyms: Dimethylarsinic acid, Hydroxydimethylarsine oxide & Dimethylarsonic acid


Potential Hazards/Toxicity

OSHA Hazards

Carcinogen, Target Organ Effect, Toxic by inhalation, Toxic by ingestion, Irritant, Teratogen



Target Organs

Kidney, Gastrointestinal tract, Skin, Heart, Brain, Bone marrow, Nerves & Liver

Pictogram



Potential Health Effects

Inhalation Toxic if inhaled. Causes respiratory tract irritation.

Skin May be harmful if absorbed through skin. Causes skin irritation.

Eyes Causes eye irritation.

Ingestion Toxic if swallowed.

Aggravated Medical Condition -

Cacodylic acid may be partially reduced to inorganic arsenic in the body. Exposure to arsenic compounds can cause:, burning, dry nose, dry mouth, Muscle cramps/spasms., May cause irritation of the:, Gastrointestinal tract, Vomiting, Nausea, Diarrhea, Shock & death.


Signs and Symptoms of Exposure: Drowsiness, Tremors, Convulsions, dry mouth, metallic taste, loss of appetite, respiratory difficulties, garlic-like breath odor, garlic-like perspiration.
Personal Protective Equipment (PPE)

Respiratory Protection

Respirators should be used only under any of the following circumstances:



  • As a last line of defense (i.e., after engineering and administrative controls have been exhausted).

  • When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will be exceeded.

  • Regulations require the use of a respirator.

  • An employer requires the use of a respirator.

  • There is potential for harmful exposure due to an atmospheric contaminant (in the absence of PEL)

  • As PPE in the event of a chemical spill clean-up process

Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S. This is a regulatory requirement. (https://www.ehs.ucla.edu/ep/ih/resp)


Hand Protection

Nitrile gloves are recommended for Cacodylic acid.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Cacodylic acid..

Refer to glove selection chart from the links below:



http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf

OR

http://www.allsafetyproducts.com/glove-selection-chart-chemical-breakthrough-ratings.html

OR

http://www.showabestglove.com/site/default.aspx

OR

http://www.mapaglove.com/


Eye Protection

Safety glasses (ANSI approved)



Skin and Body Protection

Lab coats should be worn. These laboratory coats must be appropriately sized for the individual and be buttoned to their full length. Laboratory coat sleeves must be of a sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.



Hygiene Measures

Wash hands immediately after use with soap and warm water.



Engineering Controls

Use in a chemical fume hood.



First Aid Procedures

General advice

Consult a physician. Show this safety data sheet to the doctor in attendance. Move out of dangerous area.



If inhaled

If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician.



In case of skin contact

Wash off with soap and plenty of water. Take victim immediately to hospital. Consult a physician.



In case of eye contact

Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.



If swallowed

Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician.



Special Handling and Storage Requirements

Precautions for safe handling

Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Provide appropriate exhaust ventilation at places where dust is formed.



Conditions for safe storage
Keep container tightly closed in a dry and well - ventilated place. Hygroscopic

Spill and Accident Procedure

Chemical Spill Dial 911 and x59797

Spill – Assess the extent of danger. Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1000 gms) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1000 gms) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water from the emergency eyewash station for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Medical Emergency Dial 911 or x52111



Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency – Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.

Decontamination/Waste Disposal Procedure

General hazardous waste disposal guidelines:

Label Waste

  • Affix an on-line hazardous waste tag on all waste containers using the WASTe Online Tag Program https://ehs.ucop.edu/waste as soon as the first drop of waste is added to the container

Store Waste

  • Store hazardous waste in closed containers, in secondary containment and in a designated location

  • Double-bag dry waste using transparent bags https://www.ehs.ucla.edu/hazwaste/management/containers

  • Waste must be under the control of the person generating & disposing of it

Dispose of Waste

  • Dispose of regularly generated chemical waste within 90 days

  • Call EH&S at x61887 for questions

  • Empty Containers

  • Dispose as hazardous waste if it once held extremely hazardous waste (irrespective of the container size) https://www.ehs.ucla.edu/hazwaste/types/extremely-hazardous

  • Consult waste pick-up schedule

https://www.ehs.ucla.edu/hazwaste/management/pick-ups

Prepare for transport to pick-up location



  • Check on-line waste tag

  • Write date of pick-up on the waste tag

  • Use secondary containment

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://msds.ehs.ucla.edu.



Protocol/Procedure

Quantities covered by this SOP:

0 - 13.8 grams (1M solution)

Conditions covered by this SOP:

4 °C – 37 °C

Prepare 0.5M-1M stock solution buffered at the appropriate pH (between 6-7). Stocks are made in 50 mL batches.



  • For 50 mL of a 1M solution, add 6.9g to 50 mL of water

  • Adjust pH with 0.2 N NaOH.

  • Use the pH meter in the fume hood since this is a toxic compound

  • Filter and store at 4 °C in a properly labeled bottle


Preparation of buffers containing cacodylic acid:

Add the required volume of the stock solution and mix well with the other required components for the buffer (salts, ions, reducing agents, protease inhibitors, etc.). Make sure that none of the additional reagents will adversely react with the cacodylic acid. For fixative use, dilute the stock solution in fixative until desired concentration (an example is 0.1 M cacodylic acid) is reached. Label the bottle indicating it contains cacodylate and store adequately.


NOTE

Any deviation from this SOP requires approval from PI.



Documentation of Training (signature of all users is required)

  • Prior to conducting any work with Cacodylic acid., designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.




  • The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the SDS provided by the manufacturer.




  • The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last one year.

Principal Investigator or Lab Supervisor SOP Approval

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Cacodylic acid Page of Date: 9/15/2015
UCLA- EH&S GKM/


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