I V course / educ years The plan of lectures on medical biology and genetics for the students of I course of stomatology faculty



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The plan of lectures on “Biochemistry for the students

of II course stomatology faculty (autumn term)


N

Themes

Ho­urs

1

Subjects of biochemistry, short develop history, perspectives. Develop his­to­ry of biochemistry in Azerbaijan. Chemical components of vital or­ga­­ni­sms. Chemistry, property and func­tion of carbohydrates and lipids.

2

2

Aminoacids: structure, physico-chemical properties and func­tions. Proteins-main substances of life, it’s functions and structure. Physico-chemical properties. Kind of structural or­ganizations, konformations. The main simple proteins.

2

3

Conjugates proteins: phospho-, glycol-, metalo-, lipo-, chro­mo-proteins – structure and functions. Nucleoproteins: struc­tu­re and pro­perties.

2

4

Short characteristics of enzymes: chemical nature, properties, mecha­nism of influence. Units of activity and mechanism of requlations.

2

5

Bio­che­mical characteristics of vitamins, classifications. Fatly soluble vitamins. Water soluble vitamins (B1, B2, B6, PP, B5, B12, B9) it’s structure and functions.

2

6

Vitamin C, biotin, vitamin-like substances. Antivitamines. Characte­ristics of hormones, classifications. Suites of hor­mo­nes, mecha­nism of influence.

2

7

Hypotalamus and hypophyseal hormones. Pancreas, cortical, tiroid and sex hormones, it’s structure and functions. Hor­mon-theraphy.

2

The plan of practical training on “Biochemistry for the students

of II course stomatology faculty (autumn term)


N

Themes

Hours



Acquaintance with group, department security technique ru­les, laboratory equipment and main problems static biochemistry.

4



Classification, chemistry and biological functions of carbo­hyd­rates and lipids.

Lab. work: Reactions of monosaccharides. Hyd­ro­lysis of starch. Emulsi­fi­ca­tion. Definitions of unsatu­ra­ted fatty acids. Quantity reactions of cho­lesterol .

4



Structure of amino acids, physico-chemical properties, nature of peptide bond.

Lab. work: Chromatografy of amino acids on paper. Color re­ac­ti­ons of amino acids .

4



Colloquium: Chemistry, functions of carbohydrates, lipids and ami­no acids. Passing of free themes.

2



Structure physico-chemical properties of proteins.

Lab. work: Precipitation reactions of proteins. Definitions of iso electric points of proteins. Hydrolysis of protein: Biuret reac­ti­ons.

4



Structure and functions of conjugated proteins.

Lab. work: Reactions of phospho-, qlyco- and chromo pro­teins. Hydro­lysis of nucleo­proteins.

4



Colloquium: Chemistry of proteins and nucleic acids.

2



Structure and properties of enzymes.

Lab. work: Termolability, spesifity, of optimum pH of enzymes. Kinetic of lipase ac­ti­vity.

4



Activators and inhibitors of enzymes. Regulation of enzymatic activity.

Lab. work: Definition of amylase activity by Karavey methods. Influence of activators and inhibitors on amylase activity.

2



Classification, structure and biological functions of vitamins.

Lab. work: Reactions of vitamin: A, B1, B2, B6, PP. Quality and quantity definition of vitamin C.

4



Colloquium: Enzymes and vitamins. The accepting of free themes.

2



Structure, mechanism of influence and biological functions of hormones.

Lab. work: Reactions of tiroksin, adrenalin and insulin.

4



Seminare: Hormones.

2



Colloguium: Chemical nature, mechanism of influence and biological functions of hormones. The accepting of free themes.

2



Finely balls for semester. The accepting of free themes.

2



The plan of lectures on “Dynamic and functional biochemistry

for the students of II course stomatology faculty (spring term)


N.

Themes

Hours



General pathways of katabolism (I and II pathways), its enerhetiic value. Bioligical oxidation. Structure and function of electron transporting chain (ETC).

2



Digestion and uptake of carbohydrates. Synthesis and breakdown of glycogen. Glycolysis. Aerobic glycolysis. Pentosephosphate pathways of carbohydrates oxidation. Glyconeogenesis. Carbohydrate metabolism disturbances.

2



Digestion of proteins. Conversion of aminoacids by the action of interstinal microflora. Formation of ammonia in orqanism. Mechanism it's toxicity and detoxification. Protein synthesis.

2



Synthesis and degradation of hemoglo­bin. Formation of bile pigments. Jaundices. Metabolism of nucleoproteins. Metabolism and disturbance of purine and pirimidine nucleotides.

2



Digestion of lipids. Resynthesis of lipids in intestine. Oxidation of fatty acids. Metabolism of ketone body. Synthesis of cholesterol. Disturbance of lipis metabolism. Hereditary lipidosis. Biochemistry of bone, tooth. Caries.

2



The plan of practical training on “Dynamic and functional biochemistry

for the students of II course stomatology faculty (spring term)


N

Themes

Hours

1

Introducing with thematic plan.

2

2

The basic metabolic pathways. Respiratory chain.

Experiments: Qualitative and quantitative determination of catalase.

2

3

Colloquium: The basic metabolic pathways. Energetic metabolism. Struc­ture and function if the cell membranes. Passing a free topics.

2

4

Metabolism of carbohydrates. Digestion of carbohydrates.

Glycolysis and gluconeogenesis. Diabetus mellitus.



Experiments: Determination glucose in blood by the glucosoxidase method. Sugar load method.

2

5

Colloquium: Metabolism of carbohydrates.

Passing a free topics.



2

6

Digestion and conversion of aminoacids.

Experiments: Analysis of gastric juice. Protein digestion by pepsin and trypsin.

2

7

General and specific pathways of aminoacids metabolism. Synthesis of urea. Disorders of aminoacids metabolism.

Experiments:

Determination of urea in blood. Determination of ALT and AST activity.



2

8

Colloquium: Metabolism of simple proteins and aminoacids.

Passing a free topics.



2

9

Metabolism of complex proteins. Synthesis and degradation of hemoglo­bin. Degradation of purin nucleotides.

Experiments: Determination of hemoglobin in blood. Determination of bilirubin in blood.

2

10

Colloquium: Metabolism of complex proteins. Protein synthesis. Passing a free topics.

2

11

Metabolism of lipids. Digestion of lipids. Synthesis of cholesterol. Disorders of lipid metabolism.

Experiments: Qualitative determination of bile acids.

Determination of cholesterol.



2

12

Colloquium: Metabolism of lipids.

Passing a free topics.



2

13

Chemical composition of tooth, saliva and urine.

Experiments: Analysis of urine. Determination the total proteins in blood. Determination of inorganic phosphate and Ca2+ in the tooth. Determination of rhodanides in saliva.

2

14

Colloquium: Biochemistry of blood and urine, liver, tooth and saliva.

2

15

Passing a free topics. Summary of dynamic biochemistry.

Calculation of points.



2



The plan of lectures on microbiology and immunology for

the students of stomatological faculty for winter term


N

Topics

Hours

1

Medical microbiology and immunology, its objects, development steps. Morphology, structure, classification and taxonomy of microorganisms

2

2

Physiology, nutrition, metabolism, multiplication of microorganisms

2

3

Action of physical chemical and biological factors on microorganisms. Antibiotics. Bacteriophages. Genetic of microorganisms

2

4

Microflora of environment. Normal microflora of human organism

2

5

Infection and infectious process. Virulence factors of microorganisms

2

6

Immunity, its functions. Non-specific protection factors of host. The role of oral cavity microflora on non-specific immunity

2

7

Specific (acquired) immunity. Antigens, antigenic structure of microbial cell. Antibodies, immunoglobulins, its classes

2

8

The immune system of human organism. Immune response, its types and mechanisms. Immunotherapy, immunoprevention and immunodiagnostics

1



The plan of practical training on microbiology and immunology for

the students of stomatological faculty for winter term


N

Topics

Hours

1

Microbiology laboratory. The methods of microbiology investigation. Microscopes. The working manner with microscopes

2

2

Microscopic method. Smears preparation from different pathological specimens and pure cultures. Morphology and classification of bacteria. Aniline stains. The simple stain

2

3

Structure of bacterial cell. Gram and Neisser stains

2

4

Structure of bacterial cell. Acid-fast bacteria and Ziehl Neelsen stain. Spores and spore stain

2

5

Structure of bacterial cell. Capsule. Gins-Burry stain. Burry stain. Flagella. Investigation of bacterial motility. Vital staining

2

6

Morphology and classification of Spirochetes, Rickettsiae, Chlamydia, Mycoplasma and Actinomyces (Giemsa stain)

2

7

Morphology and classification of fungi protozoa and viruses

2

8

Metabolism and nutrition of microorganisms, nutrition media. Action of physical and chemical factors on microorganisms. Sterilization and disinfection

2

9

Respiration and multiplication of microorganisms. Cultivation of aerobes and anaerobes

2

10

Bacteriological method. Isolation of the pure culture of aerobes and anaerobes (the first day)

2

11

Isolation of the pure culture of aerobes and anaerobe (the second day). Cultural character of microorganisms

2

12

Isolation of the pure culture (the third day). Biochemical character of microorganisms

2

13

Cultivation of viruses, rickettsia and chlamydia. Bacteriophages. Obtaining and studying of bacteriophages

2

14

Obtaining and classification of antibiotics. Antibiotic sensitivity tests

2

15

Ecology of microorganisms. Microflora of air, water, soil and human organism

2

16

Conclusion lesson

2

17

Infection. Laboratory animal inoculation. Determination of pathogenecity and virulence of microorganisms

2

18

Immunity. Non specific protection factors of host. Phagocytosis. Determination of phagocytes’ activity

2

19

Serological reactions. Agglutination reactions. Hemagglutination reactions, passive hemagglutination test. Coombs test

2

20

Precipitation reactions. Neutralization tests (toxin neutralization and virus neutralization tests). Lysis reactions. Complement fixation test (CFT)

2

21

Immunofluorescence method. Enzyme linked immunosorbent assay (ELISA). Radioimmunoassay method. Immunoblotting. Polymerase chain reaction (PCR)

2

22

The principles of immunoprevention and immunotherapy. The vaccine and antiserum

2

23

Conclusion lesson

1



The plan of lectures on microbiology and immunology for

the students of stomatological faculty for summer term


N

Topics

Hours

1

Introduction to basic microbiology. Pathogenic cocci and opportunistic bacteria (Klebsiella, Pseudomonas aeruginosa, Proteus)

2

2

The causative agents of intestinal infections (genus Escherichia, Shigella, Salmonella, Vibrio, Campylobacter and Helicobacter)

2

3

The causative agents of zoonotic infections (genus Yersinia, Francisella, Bruсella, Bacillus, Listeria). Pathogenic and opportunistic anaerobes (genus Clostridium and Bacteroides)

2

4

Genus Haemophilus, Bordetella, Legionella, Corynebacterium, Mycobacterium and Actinomyces

2

5

Pathogenic Spirochetes, Rickettsiae, Chlamydiae and Mycoplasma

2

6

Pathogenic fungi and protozoa

2

7

Introduction to basic virology. The causative agents of respiratory viral infections (orthomyxoviruses and paramyxoviruses). Herpes viruses, picornaviruses and rhabdviruses

2

8

Hepatitis viruses. Human immunodeficiency viruses

1


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