Department of Microbiology Quality Manual



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Department of Microbiology

Quality Manual

Policy QPCMI02001.13

Page of

Section: Process Control

Subject Title: Pre-analytical Procedure - Specimen Collection





Table of Contents


BLOOD CULTURE 5

Isolator 10 – Blood Culture System for Dimorphic Fungi 7

ENTERIC SPECIMENS 7

Faeces/Rectal Swabs 7

Rectal/Large Bowel (Colon) Biopsies 7

Duodenal or Small Bowel Aspirate / Swab / Biopsy 8

GENITAL SPECIMENS 8

Cervical Swabs 8

Group B Streptococcus Screen 8

Vaginal Swab for Vaginitis/Vaginosis Screen 8

Vaginal Swab for Culture 8

Vaginal Swab for Chlamydia / GC 9

Vaginal swab for cases of sexual abuse/ toxic shock / children 9

Urethral Swab 9

Penis Swab 9

Seminal Fluid 9

Endometrial Swabs, Biopsies and Curettings, Placenta Swab/Tissue, 9

Products of Conception, Endometrial/Uterine, Cul de sac/Transvaginal, 9

Fallopian Tube, Tubo-Ovarian Swabs or Aspirates 9

Genital Ulcer Swab 9

Intra-Uterine Device 9

INFECTION CONTROL SCREENING SPECIMENS 11

Methicillin Resistant Staphylococcus aureus (MRSA) Screen 11

Vancomycin Resistant Enterococcus (VRE) Screen 11

ESBL and Carbapenemase Screen 11

Resistant Gram Negative Bacilli 11

Klebsiella oxytoca or Klebsiella pneumonia Screen 11

Resistant Pseudomonas aeruginosa Screen 11

Group A Streptococcus Screen 12

MOLECULAR DIAGNOSTICS SPECIMENS 12

MYCOLOGY SPECIMENS 13

RESPIRATORY SPECIMENS 14

Bronchoalveolar Lavage (BAL), Bronchoscopy Aspirates/Washings – Routine 14

CMV Surveillance Bronchoscopy Specimens 14

Bronchial Brush Specimens 14

Epiglottal Swabs 14

Open Lung/Transthoracic Needle/Transbronchial Lung Biopsies/Lung Aspirates 14

Mouth Swabs 15

Nasal Swabs 15

Nasopharyngeal Swabs/Auger Suctions for Bordetella pertussis 15

Oral Abscess Swabs 15

Sinus/Antral Specimens 15

Sputum including Endotracheal tube and Tracheotomy Specimens 15

Throat Swabs 16

Gastric Aspirates/Biopsies 16

SEROLOGY SPECIMENS 16

STERILE BODY FLUID SPECIMENS 16

Cerebrospinal Fluids 16

Other Sterile Fluids – Amniotic, Pleural (Thoracentesis/Empyema), Peritoneal (Ascites), Synovial (Joint), Pericardial, Tympanocentesis, Intraocular, Hydrocele Fluids etc 16

Peritoneal Dialysis Effluent 17

Predialysis Fluid 17

Amniotic Fluids 17

Bone Marrow (Aspirates or Biopsies) 17

Blood, Platelets, and Other Transfusion Products 17

STERILITY SPECIMENS: 18

BIOLOGICAL SPECIMENS: 18

Bone Bank Specimens 18

Bone Bank Specimens - Fresh Osteochandral Allograft 18

Cardiovascular Lab Specimens (Dog) 18

Tissue Cultures Specimens for Injection 18

NON-BIOLOGICAL SPECIMENS: 18

Air Sampling by Air Flow Sampling Apparatus 18

Attest 19

Chemspore / Sterikon 19

Contact Lens & Solution 19

Distilled/De-Ionized Water Sterility 19

Environmental Monitoring 19

Hemodialysis Water Sterility 20

Miscellaneous Non-biological Samples 20

Pharmacy Sterility 20

Manufacturing Pharmacy (UHN) 20

Radiopharmacy (UHN) 21

Spore Strip 22

URINE SPECIMENS 23

Voided Urines: 23

In and Out Catheter / Catheter Insertion Urine 23

Aseptically Collected Urines 24

Segmented Urines 24

WOUND/TISSUE/ASPIRATE SPECIMENS 26

SWABS AND DRAINAGE SPECIMENS: 26

Intraoperative/Interventionial Swabs 26

Wound/Abscess Swabs and Drainage 26

Bite Wound Swabs 26

Intravenous & Central Line Catheter Exit Site Swabs 26

ABSCESS SPECIMENS (Not Swabs): 26

Intraoperative/Interventionial Abscess (Pus/Cyst Fluid or Aspirate) 26

Pus & Abscess Material (other than Intraoperative/Interventional, Rectal or Bartholin 26

Rectal Abscess 27

Bartholin’s Abscess swab/Aspirate 27

TISSUES, PROSTHETIC DEVICES, AND AUTOPSY SPECIMENS: 27

Tissues/Biopsies (other than skin or transplant tissues) 27

Skin Biopsies 27

Transplant - Bone Graft & Cadaver Fascia/Tissue/ Swab Specimens/Donor Amniotic Fluid/Membrane; Donor Corneal Ring Material 27

Prosthetic Devices (e.g. Pacemaker Wire, Dacron Graft, Prosthetic Valve) 27

Autopsy Specimens 28

CATHETER SPECIMENS: 28

Intravascular Catheter Tips 28

Peritoneal Dialysis Catheter/Canula 28

BILE SPECIMENS: 28

Bile and Bile Stents 28

MISCELLANEOUS FLUID SPECIMENS: 28

Breast Milk 29

Total Parental Nutrition (TPN) 29

EAR SPECIMENS 29

Ear Swab 29

Tympanocentesis Fluid 29

EYE SPECIMENS 29

Eye / Conjunctival / Lid Swabs 29

Eye / Corneal Scrapings 30

Intraocular Aspirates 30

Lacrimal (Tear Duct) Stone / Secretions 30

FACIAL SPECIMENS 30

Facial Swabs 30

Record of Edited Revisions 30



Specimen Rejection Criteria QPCMI06001

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