Curriculum vitae dr. Firoz anwar



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CURRICULUM VITAE

DR. FIROZ ANWAR


Name : Firoz Anwar

Nationality : Indian

Present Position : Lecturer

Department of Plant Production,

College of Food and Agricultural Science,

King Saud University

Riyadh-11451, Kingdom of Saudi Arabia

E.mail: anfiroz@gmail.com



______________________________________________________________________________

Educational Qualification


  • Ph. D. in Biotechnology from University of Delhi, New Delhi, India, 2007. Grade A+

  • M.Sc. Biotechnology (1st Division) from L.N.M. University, Darbhanga, India, 2000. Grade A+

  • M.Sc. Chemistry (Org. Spl.) (1st Division) from L.N.M. University, Darbhanga, India, 1995. Grade A

  • Graduation in, Chemistry (Hons), (1st Division) Botany and Zoology from L.N.M. University, Darbhanga, India, 1993. Grade A+

Academic Positions and Experience


March 2009-till date : Lecturer, Department of Plant Production, College of Food and Agricultural Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.

June 2008-March 2009 : R. Scientist, Plant Molecular Biology, University of Delhi, New Delhi, India.

September 2005-Feb. 2008 : Senior Researcher, University of Delhi, New Delhi, India.

April 2001-August 2005 : Senior Research fellow, University of Delhi, New Delhi, India

Dec. 2000-March2001 : Lecturer, Department of Physiology and Biochemistry, Mithila Minority Dental College and Hospital, Darbhanga, India.

June 1995-July 1997 : Lecturer, Department of Chemistry, Q. A. Inter College, Jale, Darbhanga, India.

Research Grants Received/Under Process


  1. Grant No. 10-BIO1119-02. Co-Principal investigator of the running self design research project entitled “Salt Tolerant Genes from Atriplex halimus L.” approved from KACST, NPST, Kingdom of Saudi Arabia (2010--2012).

  2. Principal investigator of the self design research project entitled “Standardization of regeneration and genetic transformation systems in Chickpeasubmitted to KACST, NPST, Kingdom of Saudi Arabia (2011-2013).

  3. Co-Principal investigator of the self design research project entitled “Genetic diversification of faba bean germplasm through mutation and tissue culture techniques” submitted to KACST, NPST, Kingdom of Saudi Arabia (2011-2013).

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TECHNICAL EXPERTISE


Genetic Engineering Tech.

  • E. coli & Yeast culture, Transformation, Isolation of plasmid DNA, Purification of plasmid DNA, Restriction Digestion & Mapping, Gel Electrophoresis, Isolation of Genomic DNA, Native & SDS-PAGE, PCR, Southern blotting, ELISA, Western-blotting, DNA sequencing, FISH, Human Blood culture

Plant Tissue Culture Tech.

  • Preparation of different Culture Media, Isolation of Protoplast, Culture & Regeneration and genetic transformation of Chickpea (Cicer arietinum) Mustard (Brassica juncia), Faba bean (Vicia faba L.), Groundnut, Sorghum, Rice etc.

Biochemical & Biophysical Tech.

  • Protein purification tech. (Gel Exclusion & Ion Exchange Chromatography)

  • Measurement of Photosynthetic processes; Extraction & Estimation of the Photosynthetic Pigments (chlorophyll a, b and carotenoid), Isolation of Thylakoid Membrane, Isolation of Mitochondria, Assay of Photochemical Activities by Clark-type Electrode, Handy PEA (Plant Efficiency Analyser, Hansatech, UK); PS1, PS2 & Whole Chain Dependent Electron Transport Activities etc.

  • Relative water Content, Leaf Water Potential (Water potential of the leaves measured by PSYPRO water potential system (WESCOR, INC. USA)

  • Antioxidant Enzyme; Catalase, Peroxidase, Superoxide dismutase, Ascorbate peroxidase, Monodehydroascorbate reductase, Dehydroascorbate reductase and Glutathione reductase

Estimations & Quantification

  • Sugars, Amino acids, glycine betaine, Imino acid like Proline, MDA (free radicals), H2O2

Cytogenetics Techniques

  • Metaphase chromosome preparation., G-banding, Karyotypic analysis


P.G. Research Guided/Supervised : 02



Awards and Honors


  • National fellowship from 4th to 10th.

  • Senior Research Fellowship awarded by DBT Govt. of India and ISCB Switzerland during the Ph.D. Programmed, 2001-2005.

Associate Editor


Env. & We International Journal for Science & Technology, Hisar, India, since November, 2009.

Invited Reviewer


Journal of Agricultural and Food Chemistry (I F ~2.5)

Journal of Environmental Biology (I F ~1.35)

Plant Cell Tissue and Organ Culture

African Journal of Biotechnology


Publications (International Research Paper and Review Articles)


  • Sharmila, P., Gupta, S., Anwar, F. and Pardha Saradhi, P. (2006). Introduction of Glycinebetaine through gentic engineering enhances potential of plants to withstand abiotic stresses. In: Current Concept in Botany. Mukherji, K.G. and Manoharachary, C. (Ed.), I.K. International Group. USA. pp 471-491.

  • Sharmila, P., Anwar, F., Sharma, K.R. and P. Pardha Saradhi (2008). Management of Abiotic Stresses in grain legumes through manipulation of genes for compatible solutes. In: P.B. Kirti (Ed.), Handbook of New Technologies for Genetic Improvement of Legumes, CRC Press, Boca Raton, USA. 2008, pp. 577–603. https://bookweb.kinokuniya. co.jp/htmy/1560223081. htmlChapter

  • Anwar, F., Sharmila, P. and Pardha Saradhi, P. (2008). An ideal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea. Physiology and Molecular Biology of Plants (Springer) 14: 329-335. (Impact ~)

  • Sharmila, P., Phanindra, M.L.V., Anwar, F., Singh, K., Gupta, S. and Pardha Saradhi, P. (2009). Targeting prokaryotic choline oxidase into chloroplasts enhance the potential of photosynthetic machinery of plants to withstand oxidative damage. Plant Physiology and Biochemistry (ELSEVIER). 47: 391-396. (Impact ~2)

  • Anwar, F., Sharmila, P. and Pardha Saradhi, P. (2009). No more hurdle: In-vitro chickpea rooting and cent-percent transplantation. Australian journal of Basic and Applied Science. 3(3): 2491-2496.

  • Khan, S., Fahad, A.Q., Anwar, F. (2009). Sodium Azide: a Chemical Mutagen for Enhancement of Agronomic Traits of Crop Plants. Environ. We. International Journal of Science & Technology, 4: 1-21.

  • Khan, S., Mirza, K. Z., Anwar, F. and Malik Zainul Abdin (2010). Development of RAPD markers for authentication of Piper nigrum (L.). Environ. We. International Journal of Science & Technology. Published.

  • Anwar, F., Sharmila, P. and Pardha Saradhi, P. (2010). No more recalcitrant: Chickpea regeneration and genetic transformation. African Journal of Biotechnology. 9(6): 782-797. (Impact ~0.56).

  • Anwar F., Salem S. Alghamdi, Ammar HM and Siddique, KHM (2011). An efficient in vitro regeneration protocol for faba bean (Vicia faba L.) Accepted for Publication (ISI Journal).

  • Anwar, F., Alghamdi, S.S., Salim Khan, Abhishek Chandra and Migdadi, H. (2011). Germplasm selection, collection, conservation and evaluation of legumes and cereals: Review on Awareness. Being communicated to ISI Journal.

Abstract Publications and Presentations (International) 11


  • Firoz Anwar, P. Sharmila, K. Singh, M.L.V. Phanindra and P. Pardha Sardhi (2008). The potential of codA chickpea transgenic genotypes to withstand abiotic stresses is linked to their stronger antioxidant system. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 4, Issue 1.

  • Sharmila, P., Firoz Anwar and P. Pardha Sardhi (2008). Transgenic chickpea expressing the codA gene exhibited superior agronomic traits and enhanced potential to withstand drought and low temperature. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 4, Issue 1.

  • Sharmila, P., Firoz Anwar and Pardha Saradhi, P. (2008). Targeting prokaryotic choline oxidase into chloroplasts enhance the potential of photosynthetic machinery of plants to withstand photoinhibition. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 4, Issue 1.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2004). Successful expression of bacterial choline oxidase genein chickpea. 3nd pulse Internationl Network Meeting. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 3, Issue 1.

  • Jodha Elangbam, Firoz Anwar, P. Sharmila and P. Pardha Sardhi (2003). Transformation of chickpea with the codA gene. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 2, Issue 1.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila, Kiran K. Sharma and P. Pardha Sardhi (2002). Standardization of protocol for healthy rooting. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 3.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2002). Transformation of chickpea with the codA gene. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 3.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2002). Transformation of chickpea with the codA gene to enhance its tolerance to abiotic stress. 2nd pulse Internationl Network Meeting. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 3.

  • Kiran K. Sharma, Reto J. Strasser, P. Sharmila, Firoz Anwar and P. Pardha Sardhi (2002). Enhancing tolerance of chickpea to draught, freezing and low temperature through genetic engineering with the codA and P5CSF-129A gene. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 3.

  • Sharmila, P., Firoz Anwar and Pardha Sardhi (2002). Transformation of chickpea (Cicer arietinum L.) with agrobacterium. Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 2.

  • Pardha Sardhi, P., Sharmila,P. and Firoz Anwar (2001). Refining protocol for efficient regeneration of chickpea to be used for transforming with the codA gene to enhance its tolerance to draught. First Annual International pulse meeting at Jorhat, Indo-Swiss Collaboration in Biotechnology Pulse Network News Letter, Volume 1, Issue 1.

Poster Presentations (International) 05


  • Firoz Anwar, P. Sharmila, K. Singh, M.L.V. Phanindra and P. Pardha Sardhi (2008). The potential of codA chickpea transgenic genotypes to withstand abiotic stresses is linked to their stronger antioxidant system.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2004). Successful expression of bacterial choline oxidase gene in chickpea.

  • Jodha Elangbam, Firoz Anwar, P. Sharmila and P. Pardha Sardhi (2003). Transformation of chickpea with pGAH/pSG with the codA gene.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2002). Transformation of chickpea with the codA gene to enhance its tolerance to abiotic stress.

  • Firoz Anwar, Jodha Elangbam, P. Sharmila and P. Pardha Sardhi (2001). Standardization of chickpea regeneration and transformation of chickpea with the codA gene.

  • Firoz Anwar and Salem S. Alghamdi (2010). An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of fava bean (Vicia faba L.).

  • Monther T. Sadder, Abdullah A. Al-Doss, Firoz Anwar and Mohammad Al-Thamra (2011). Salt Tolerant Transcriptome from Atriplex halimus. Canada.

Seminars/Conferences


  • Active participant of Indo-German co-operation program in Science and Technology on “Agriculture and Environmental Biotechnology” at University department of Biotechnology, L.N. Mithila University, Darbhanga (Bihar).

  • Active participant of seminar on “Perspective of wetlands” at University Department of Botany L.N. Mithila University, Darbhanga (Bihar) by DST and CSIR New Delhi.

  • Active participant of National Conference on “Prospect of Environmental Research in 21st century” at J. N. College, Madhubani (Bihar) by UGC Calcutta and CSIR New Delhi.

  • Active participant of “Awareness program on Hepatitis-B Vaccine” at Jamia Millia Islamia, New Delhi by UGC and CSIR New Delhi.

  • Active participant of National Conference on “Intellectual Property Rights” at Jamia Millia Islamia New Delhi by UGC and CSIR New Delhi.

  • Active participant of National Conference on “How safe is: Drinking water” at Department of Environmental Biology North Campus University of Delhi by UGC and CSIR New Delhi.

  • Active participant of National Seminar on “Relevance of Botany in the Conservation and Improvement of Plants” at Department of Botany, University of Delhi. Delhi-7.

  • Active participant of International Seminar on “DNA Research: A Gateway to knowledge Economy” at College of Science, King Saud university, KSA. etc.

Research and Teaching Summary


  • Lecturer; I’m presently working as Lecturer, College of Food and Agricultural Science, Plant Production, Biotechnology Division, KSU, KSA (March 2009 to till date) and actively involved in Research and Teaching as well as inspire students to explore in unexplored areas and unearth new vistas by inventing and coining ideas which may prove landmark for generations to come.

I am also initiated research work as Co-Principal Investigator in self deigned research project in king Saud University;

1. Cloning Salt Tolerant Genes from Atriplex halimus



2. Standardization of Chickpea Regeneration and Comparative Bio-physiochemical Analysis

  • R. Scientist, PMB, University of Delhi, Delhi- 110021, INDIA (June 2008 – March 2009). I have been associated with various universities and institutions, I have assigned with the teaching theory and practical classes of variety of areas from Plant Molecular Biology and Biotechnology and establishment of Research Lab for fully workable condition. Standardized rapid and reproducible rice regeneration and transformation protocol. Construction/Cloning of barnase/barstar vectors with rice tapetum specific promoter and transformed into hyper virulent Agrobacterium stain. These vectors used for the transformation of rice for the production of hybrid vigour/heterosis to produced high yielding varieties.

  • Senior Researcher; Biotechnology Lab., Department of Environmental Biology, University of Delhi, Delhi, India Four Years and five months Research Experience as Senior Research Fellow (Funded by Department of Biotechnology, India and Indo Swiss Collaboration in Biotechnology, Switzerland) under Prof P. P. Saradhi in the field of Molecular Biology/Biotechnology and Genetic Engineering. (From April 2001 to Feb. 2008) and regularly taking M. Sc. (Environmental Biology) practical and theory classes on Biotechnology/Molecular Biology and also help M. Sc. Project trainees for research, compilation and completion (Research oriented problem solving) of M. Sc. Project during the completion of my Ph. D. till 2007.

IPR issues/proprietary rights is a matter of concern therefore, the codAPS gene isolated from a strain of Arthrobacter sp. (IMTECH, Chandigarh) was used for transforming chickpea to enhance its tolerance to abiotic stresses. Successfully fine-tuned regeneration and transformation protocols and developed codAPS transgenic genotypes of chickpea which showed enhanced tolerance to salinity, drought, low temperature and frost with a superior anti-oxidant system. Another unique observation made during research study was the increase in the capacity of codAPS lines to accumulate proline compared to wild type. The findings presented in my PhD thesis supported by publications like any other scientific investigation while providing answers to some pre-existing questions has also opened up new vistas to be pursued in future.

  • I have been worked with Dr. K.K. Sharma (Principal Scientist), Genetic Transformation Lab, International Crop Research Institute in Semi Arid Tropical Region (ICRISAT), Patancheru, Hyderabad; Dr. M. B. Chetti (Prof. and Head), Dept. of Plant Physiology, UAS, Dharwad (2002-2004) and Dr. S. Yadav (Principal Scientist) and Dr. Jitendra Kumar (Principal Scientist), Legume and Pulse Research Centre, Division of Genetics, IARI, PUSA, Delhi, India.

  1. Successfully developed chickpea transgenic plants by introducing codA as well as P5CSF129A gene to enhanced abiotic stress tolerance at GTL, ICRISAT, Hyderabad.

  2. To help and train researchers of GTL Lab, ICRISAT, Hyderabad for estimation of proline and free radical in P5CSF129A chickpea transgenic plants.

  • M.Sc. Project Trainee, Prof. MJ Raman, Prof. K Lakhotia and Prof. BD Singh, Department of Zoology and Biotechnology, Banarus Hindu University, Varanasi, UP to get trained in Plant Tissue Culture, Cell and Molecular Biology Techniques and also tested; Human Y chromosome specific sequence tagged site confirmation for sterility test.

  • Dec. 2000-March2001 : Lecturer, Department of Physiology and Biochemistry, Mithila Minority Dental College and Hospital, Darbhanga, India.

  • June 1995-July 1997 : Lecturer, Department of Chemistry, Q. A. Inter College, Jale, Darbhanga, India.



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