Appendix 2-5: Rejected ecotox bibliography for Chlorpyrifos



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Oryzaephilus surinamensis (L.), are presumed to play a role in conferring resistance to chlorpyrifos-methyl. Colorimetric assays using substrates of p-nitrophenyl acetate, alpha-naphthyl acetate and beta-naphthyl acetate showed 4.8, 7.8 and 7.5 times higher carboxylesterase hydrolytic activities in VOSCM than those in VOS48, an organophosphorus insecticide-susceptible strain. Carboxylesterase zymograms showed different banding patterns between VOSCM and VOS48. A primary carboxylesterase in the VOSCM strain, not detected in VOS48, was purified and characterised by chromatographic and electrophoretic techniques. On the basis of native and SDS-polyacrylamide gel electrophoresis, the molecular mass of the purified carboxylesterase from VOSCM was 120 kDa and consisted of two 60 kDa subunits. The purified carboxylesterase activity was totally inhibited by 10(-1) mM chlorpyrifos-methyl and by 10(-3) mM chlorpyrifps-methyl oxon. The purified enzyme did not hydrolyse insecticide substrates. Therefore, these results indicate that the purified carboxylesterase may play an important role in chlorpyrifos-methyl detoxification by sequestration.
Keywords: carboxylesterase, chlorpyrifos-methyl, Oryzaephilus surinamensis,
ISI Document Delivery No.: 766YB

764. Lein, P. J.; Bonner, M. R.; Farahat, F. M.; Olson, J. R.; Rohlman, D. S.; Fenske, R. A.; Lattal, K. M.; Lasarev, M. R.; Galvin, K.; Farahat, T. M., and Anger, W. K. Experimental strategy for translational studies of organophosphorus pesticide neurotoxicity based on real-world occupational exposures to chlorpyrifos. 2012; 33, (4): 660-668.


Rec #: 63629
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: Translational research is needed to understand and predict the neurotoxic consequences associated with repeated occupational exposures to organophosphorus pesticides (OPs). In this report, we describe a research strategy for identifying biomarkers of OP neurotoxicity, and we characterize pesticide application workers in Egypt's Menoufia Governorate who serve as our anchor human population for developing a parallel animal model with similar exposures and behavioral deficits and for examining the influence of human polymorphisms in cytochrome P450 (CYP) and paraoxonase 1 (PON1) enzymes on OP metabolism and toxicity. This population has previously been shown to have high occupational exposures and to exhibit a broad range of neurobehavioral deficits. In addition to observational studies of work practices in the field, questionnaires on demographics, lifestyle and work practices were administered to 146 Egyptian pesticide application workers applying pesticides to the cotton crop. Survey results indicated that the application workforce uses standard operating procedures and standardized equipment provided by Egypt's Ministry of Agriculture, which provides a workforce with a stable work history. We also found that few workers report using personal protective equipment (PPE), which likely contributes to the relatively high exposures reported in these application workers. In summary, this population provides a unique opportunity for identifying biomarkers of OP-induced neurotoxicity associated with occupational exposure. (C) 2012 Elsevier Inc. All rights reserved.
Keywords: Biomarkers, Chlorpyrifos, Occupational exposure, Organophosphorus
ISI Document Delivery No.: 990FU

765. Leoni, Claudia; Balduzzi, Maria; Buratti, Franca Maria, and Testai, Emanuela. The contribution of human small intestine to chlorpyrifos biotransformation. 2012 Nov 23-; 215, (1): 42-48.


Rec #: 1040
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Despite the oral intake is the major route of exposure to chlorpyrifos for the general population, few data are available on human intestine biotransformation. In this study the contribution of chlorpyrifos (CPF) metabolism in human small intestine was investigated in microsomes from duodenum (HDM) and ileum/jejunum (HS2M) from 11 individual donors. Samples were characterized for testosterone hydroxylated metabolite formation and CYP content quantification by means of Western blotting. The two methods gave consistent results, evidencing the presence of CY3A4 and its-related activity in 10/11 samples, among which one showed also the presence of CYP2C9. Analogously, although with high interindividual variability (about 10 fold), CPF bioactivation to chlorpyrifos-oxon (CPFO) was observed in 10/11 HDM: intrinsic clearance highest value was 0.75 pmol CPFO/(mg protein min ++M). Detoxication to 3,5,6-trichloropyrin-2-ol formation was negligible. The comparison between HDM and HS2M indicates that most CPF bioactivation was confined in the duodenum, declining toward the distal ileum. Results suggest that following oral exposure, the small intestine CPF bioactivation, although much lower when compared to the total hepatic metabolism, could play a role in the pre-systemic CPF clearance, with CPFO transported into the lumen by the efflux P-glycoprotein and further metabolized by esterases. Organophosphorothionate pesticide/ Chlorpyrifos/ CYP3A4/ Human small intestine metabolism

766. Lesmes-Fabian, C.; Garcia-Santos, G.; Leuenberger, F.; Nuyttens, D., and Binder, C. R. Dermal exposure assessment of pesticide use: The case of sprayers in potato farms in the Colombian highlands. 2012; 430, 202-208.


Rec #: 63689
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: Quantifying dermal exposure to pesticides in farming systems in developing countries is of special interest for the estimation of potential health risks, especially when there is a lack of occupational hygiene regulations. In this paper we present the results of a dermal exposure assessment for the potato farming system in the highlands of Colombia, where farmers apply pesticides with hand pressure sprayers without any personal protective equipment. The fractioning of the pesticide, in terms of potential and actual dermal exposure, was determined via the whole-body dosimetry methodology. using the tracer uranine as pesticide surrogate, and luminescence spectrometry as analytical method. We assessed the three activities involved in pesticide management: preparation, application, and cleaning; analyzed three types of nozzles: one with a standard discharge and two modified by farmers to increase the discharge; and derived the protection factor given by work clothing. Our results suggest that to reduce the health risk, three aspects have to be considered: (i) avoiding the modification of nozzles, which affects the droplet size spectrum and increases the level of dermal exposure; (ii) using adequate work clothing made of thick fabrics, especially on the upper body parts; and (iii) cleaning properly the tank sprayer before the application activity. (C) 2012 Published by Elsevier B.V.
Keywords: Occupational hygiene, Pesticides, Developing countries, Potato, Hand
ISI Document Delivery No.: 976KO

767. Leticia, a G; Gerardo, G B, and Leticia, A G. Determination of Esterase Activity and Characterization of Cholinesterases in the Reef Fish Haemulon Plumieri. 2008 Nov; 71, (3): 787-797.


Rec #: 45439
Keywords: SURVEY
Notes: Chemical of Concern: CPY
Abstract: Abstract: White grunt (Haemulon plumieri) has been proposed by the Mesoamerican Barrier Reef System (MBRS) Synoptic Monitoring Program as a bioindicator species. It is in this sense that the present study has a main goal to evaluate this organism's suitability as an indicator species. Individuals were captured during three seasons at the port of Sisal, Yucatan, Mexico which is located in an area that is considered to be weakly impacted by human activities such as agriculture or industry. Both cholinesterase (ChE) and carboxylesterase (CbE) activities were measured in brain, muscle, liver and eye of sampled individuals. Results indicated that ChE and CbE activities were greatest in the brain (256.3+/-43) and in the liver (191+/-21), respectively. Furthermore, ChEs detected in brain, liver and muscle were characterized, and results suggested that the acetylcholinesterase (AChE) type was more abundant relative to pseudocholinesterase (BChE) which was rare. In addition, K sub(m) and V sub(m) sub(a) sub(x) and IC sub(5) sub(0) values were calculated from the Michaelis-Menten equation. Finally, an additional experiment in vitro showed a significant decrease in both ChE and CbE activities when different tissues were exposed to model xenobiotics, such as benzo[a]pyrene and Chlorpyrifos. In conclusion, findings from this study confirm the potential suitability of H. plumieri as an organic pollution bioindicator species, and thus of practical use for environmental biomonitoring purposes.
Keywords: Agriculture
Keywords: Reefs
Keywords: Eye
Keywords: Acetylcholinesterase
Keywords: esterase
Keywords: Man-induced effects
Keywords: Xenobiotics
Keywords: Cholinesterase
Keywords: Barrier reefs
Keywords: Models
Keywords: Oceanic Abstracts; ASFA 1: Biological Sciences & Living Resources; Toxicology Abstracts
Keywords: Aromatic hydrocarbons
Keywords: biomonitoring
Keywords: Cholinesterase inhibitors
Keywords: X 24330:Agrochemicals
Keywords: Pollution
Keywords: Mathematical models
Keywords: Q1 01341:General
Keywords: Muscles
Keywords: Brain
Keywords: Carboxylesterase
Keywords: Enzymes
Keywords: Chlorpyrifos
Keywords: O 4095:Instruments/Methods
Keywords: Mexico
Keywords: ASW, Mexico, Yucatan
Keywords: Pesticides
Keywords: Liver
Keywords: Benzo(a)pyrene
Keywords: Reef fish
Keywords: Indicator species
Keywords: Haemulon plumieri
Date revised - 2008-11-01
Language of summary - English
Location - Mexico; ASW, Mexico, Yucatan
Pages - 787-797
ProQuest ID - 19312024
SubjectsTermNotLitGenreText - Pesticides; Brain; Aromatic hydrocarbons; Man-induced effects; Enzymes; Cholinesterase inhibitors; Reef fish; Barrier reefs; Indicator species; Agriculture; Reefs; Mathematical models; Eye; Acetylcholinesterase; esterase; Muscles; Carboxylesterase; Xenobiotics; Cholinesterase; Models; Chlorpyrifos; Liver; biomonitoring; Benzo(a)pyrene; Pollution; Haemulon plumieri; Mexico; ASW, Mexico, Yucatan
Last updated - 2011-12-14
British nursing index edition - Ecotoxicology and Environmental Safety [Ecotoxicol. Environ. Saf.]. Vol. 71, no. 3, pp. 787-797. Nov 2008.
Corporate institution author - Leticia, A G; Gerardo, G B
DOI - MD-0008815657; 8578926; CS0866991; 0147-6513 English

768. Levin, E.; Timofeeva, O.; Seidler, F., and Slotkin, T. Long-Term Cognitive Effects of Low-Level Developmental Organophosphate Pesticide Exposure: Divergent Effects of Chlorpyrifos, Diazinion and Parathion. 2008; 30, 251-(ABS).


Rec #: 190
Keywords: ABSTRACT
Call Number: NO ABSTRACT (CPY,DZ)
Notes: Chemical of Concern: CPY,DZ,EPRN,PRN

769. Levin, E. D.; Aschner, M.; Heberlein, U.; Ruden, D.; Welsh-Bohmer, K. A.; Bartlett, S.; Berger, K.; Chen, L.; Corl, A. B.; Eddins, D.; French, R.; Hayden, K. M.; Helmcke, K.; Hirsch, H. V. B.; Linney, E.; Lnenicka, G.; Page, G. P.; Possidente, D.; Possidente, B., and Kirshner, A. Genetic aspects of behavioral neurotoxicology. 2009; 30, (5): 741-753.


Rec #: 63729
Keywords: REVIEW
Notes: Chemical of Concern: CPY
Abstract: Abstract: Considerable progress has been made over the past couple of decades concerning the molecular bases of neurobehavioral function and dysfunction. The field of neurobehavioral genetics is becoming mature. Genetic factors contributing to neurologic diseases such as Alzheimer's disease have been found and evidence for genetic factors contributing to other diseases such as schizophrenia and autism are likely. This genetic approach can also benefit the field of behavioral neurotoxicology. It is clear that there is substantial heterogeneity of response with behavioral impairments resulting from neurotoxicants. Many factors contribute to differential sensitivity, but it is likely that genetic variability plays a prominent role. Important discoveries concerning genetics and behavioral neurotoxicity are being made on a broad front from work with invertebrate and piscine mutant models to classic mouse knockout models and human epidemiologic studies of polymorphisms. Discovering genetic factors of susceptibility to neurobehavioral toxicity not only helps identify those at special risk, it also advances our understanding of the mechanisms by which toxicants impair neurobehavioral function in the larger population. This symposium organized by Edward Levin and Annette Kirshner, brought together researchers from the laboratories of Michael Aschner, Douglas Ruden, Ulrike Heberlein, Edward Levin and Kathleen Welsh-Bohmer conducting studies with Caenorhabditis elegans, Drosophila, fish, rodents and humans studies to determine the role of genetic factors in susceptibility to behavioral impairment from neurotoxic exposure. (C) 2009 Elsevier Inc. All rights reserved.
Keywords: Genetics, Behavioral neurotoxicology, C. elegans, Drosophilia,
ISI Document Delivery No.: 513AC

770. Levin, Edward. Using zebrafish to investigate persisting cognitive and sensorimotor effects of early developmental chlorpyrifos exposure. 2010 Jul; 32, (4): 503.


Rec #: 2460
Keywords: ABSTRACT
Notes: Chemical of Concern: CPY

771. Levin, Edward; Cauley, Marty; Johnson, Joshua; Sexton, Hannah; Gordon, Karen; Seidler, Frederic, and Slotkin, Theodore. Does pharmacotherapy of preterm labor sensitize the brain to neurotoxicants? Sequential exposure to dexamethasone and chlorpyrifos. 2012 May; 34, (3): 368.


Rec #: 2630
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY

772. Li, A. A.; Levine, T. E.; Burns, C. J., and Anger, W. K. Integration of epidemiology and animal neurotoxicity data for risk assessment. 2012; 33, (4): 823-832.


Rec #: 63759
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: Most human health risk assessments are based on animal studies that can be conducted under conditions where exposure to multiple doses of a single chemical can be controlled. Data from epidemiology studies also provide valuable information about human exposure and response to pesticides. Human studies have the potential of evaluating neurobehavioral and other outcomes that may be more difficult to evaluate in animals. The human data together with animal data can contribute to a weight-of-evidence analysis in the characterization of human health risks. Epidemiology data do, however, pose challenges with respect to characterizing human health risks. Similarly, animal data at high doses or routes of exposure not typical for humans also pose challenges to dose-response evaluations needed for risk assessments. This paper summarizes some of the presentations given at a symposium held at the Xi'an, China, International Neurotoxicology Conference held in June 2011. This symposium brought together scientists from government, industry and academia to discuss approaches to evaluating and conducting animal and human neurotoxicity studies for risk assessment purposes, using the pesticides paraquat and chlorpyrifos as case studies. (C) 2012 Elsevier Inc. All rights reserved.
Keywords: Chlorpyrifos, Pesticides, Organophosphates, Neurotoxicity, Epidemiology,
ISI Document Delivery No.: 990FU

773. Li, Abby a; Lowe, Kimberly a; Mcintosh, Laura J; Mink, Pamela J, and Li, Abby A. Evaluation of Epidemiology and Animal Data for Risk Assessment: Chlorpyrifos Developmental Neurobehavioral Outcomes. 2012 Feb 1; 15, (2): 109-184.


Rec #: 39049
Keywords: REVIEW
Notes: Chemical of Concern: CPY
Abstract: Abstract: Developmental neurobehavioral outcomes attributed to exposure to chlorpyrifos (CPF) obtained from epidemiologic and animal studies published before June 2010 were reviewed for risk assessment purposes. For epidemiological studies, this review considered (1) overall strength of study design, (2) specificity of CPF exposure biomarkers, (3) potential for bias, and (4) Hill guidelines for causal inference. In the case of animal studies, this review focused on evaluating the consistency of outcomes for developmental neurobehavioral endpoints from in vivo mammalian studies that exposed dams and/or offspring to CPF prior to weaning. Developmental neuropharmacologic and neuropathologic outcomes were also evaluated. Experimental design and methods were examined as part of the weight of evidence. There was insufficient evidence that human developmental exposures to CPF produce adverse neurobehavioral effects in infants and children across different cohort studies that may be relevant to CPF exposure. In animals, few behavioral parameters were affected following gestational exposures to 1 mg/kg-d but were not consistently reported by different laboratories. For postnatal exposures, behavioral effects found in more than one study at 1 mg/kg-d were decreased errors on a radial arm maze in female rats and increased errors in males dosed subcutaneously from postnatal day (PND) 1 to 4. A similar finding was seen in rats exposed orally from PND 1 to 21 with incremental dose levels of 1, 2, and 4 mg/kg-d, but not in rats dosed with constant dose level of 1 mg/kg-d. Neurodevelopmental behavioral, pharmacological, and morphologic effects occurred at doses that produced significant brain or red blood cell acetylcholinesterase inhibition in dams or offspring.
Keywords: Risk assessment
Keywords: H 6000:Natural Disasters/Civil Defense/Emergency Management
Keywords: Data processing
Keywords: Acetylcholinesterase
Keywords: Erythrocytes
Keywords: Guidelines
Keywords: Brain
Keywords: Weaning
Keywords: Risk Abstracts; Environment Abstracts; Health & Safety Science Abstracts; Toxicology Abstracts
Keywords: Offspring
Keywords: Children
Keywords: biomarkers
Keywords: ENA 02:Toxicology & Environmental Safety
Keywords: Chlorpyrifos
Keywords: Rats
Keywords: Epidemiology
Keywords: Dams
Keywords: Reviews
Keywords: Pesticides
Keywords: Neurotoxicity
Keywords: R2 23060:Medical and environmental health
Keywords: Progeny
Keywords: X 24330:Agrochemicals
Keywords: Infants
Date revised - 2012-12-01
Language of summary - English
Pages - 109-184
ProQuest ID - 1257787020
SubjectsTermNotLitGenreText - Risk assessment; Data processing; Acetylcholinesterase; Erythrocytes; Brain; Weaning; Children; biomarkers; Chlorpyrifos; Epidemiology; Reviews; Progeny; Infants; Rats; Dams; Guidelines; Pesticides; Neurotoxicity; Offspring
Last updated - 2013-02-08
British nursing index edition - Journal of Toxicology and Environmental Health, Part B: Critical Reviews [J. Toxicol. Environ. Health, Pt. B Crit. Rev.]. Vol. 15, no. 2, pp. 109-184. 1 Feb 2012.
Corporate institution author - Li, Abby A; Lowe, Kimberly A; McIntosh, Laura J; Mink, Pamela J
DOI - 2a999589-e82f-43c4-8ed5mfgefd109; 17487701; 1093-7404; 1521-6950 English

774. Li, Bin; Ricordel, Ivan; Schopfer, Lawrence M; Baud, Frederic; Megarbane, Bruno; Masson, Patrick; Lockridge, Oksana, and Li, Bin. Dichlorvos, Chlorpyrifos Oxon and Aldicarb Adducts of Butyrylcholinesterase, Detected by Mass Spectrometry in Human Plasma Following Deliberate Overdose. 2010 Aug 1; 30, (6): 559-565.


Rec #: 40439
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: The goal of this study was to develop a method to detect pesticide adducts in tryptic digests of butyrylcholinesterase in human plasma from patients poisoned by pesticides. Adducts to butyrylcholinesterase in human serum may serve as biomarkers of pesticide exposure because organophosphorus and carbamate pesticides make a covalent bond with the active site serine of butyrylcholinesterase. Serum samples from five attempted suicides (with dichlorvos, Aldicarb, Baygon and an unknown pesticide) and from one patient who accidentally inhaled dichlorvos were analyzed. Butyrylcholinesterase was purified from 2 ml serum by ion exchange chromatography at pH 4, followed by procainamide affinity chromatography at pH 7. The purified butyrylcholinesterase was denatured, digested with trypsin and the modified peptide isolated by HPLC. The purified peptide was analyzed by multiple reaction monitoring in a QTRAP 4000 mass spectrometer. This method successfully identified the pesticide-adducted butyrylcholinesterase peptide in four patients whose butyrylcholinesterase was inhibited 60-84%, but not in two patients whose inhibition levels were 8 and 22%. It is expected that low inhibition levels will require analysis of larger serum plasma volumes. In conclusion, a mass spectrometry method for identification of exposure to live toxic pesticides has been developed, based on identification of pesticide adducts on the active site serine of human butyrylcholinesterase.
Keywords: High-performance liquid chromatography
Keywords: Suicide
Keywords: Mass spectrometry
Keywords: Environment Abstracts; Pollution Abstracts; Toxicology Abstracts
Keywords: Mass spectroscopy
Keywords: pH effects
Keywords: Ion exchange
Keywords: X 24330:Agrochemicals
Keywords: Dichlorvos
Keywords: Serine
Keywords: suicide
Keywords: pH
Keywords: Bioindicators
Keywords: Trypsin
Keywords: Chromatography
Keywords: Adducts
Keywords: dichlorvos
Keywords: Aldicarb
Keywords: Pesticides (carbamates)
Keywords: biomarkers
Keywords: ENA 02:Toxicology & Environmental Safety
Keywords: Chlorpyrifos
Keywords: Affinity chromatography
Keywords: Overdose
Keywords: Pesticides
Keywords: P 1000:MARINE POLLUTION
Date revised - 2011-05-01
Language of summary - English
Pages - 559-565
ProQuest ID - 869591083
SubjectsTermNotLitGenreText - High-performance liquid chromatography; Trypsin; Adducts; Suicide; Aldicarb; Pesticides (carbamates); biomarkers; Mass spectroscopy; Affinity chromatography; Chlorpyrifos; Overdose; Pesticides; pH effects; Ion exchange; Dichlorvos; Serine; Bioindicators; Chromatography; dichlorvos; Mass spectrometry; suicide; pH
Last updated - 2012-03-29
British nursing index edition - Journal of Applied Toxicology [J. Appl. Toxicol.]. Vol. 30, no. 6, pp. 559-565. 1 Aug 2010.
Corporate institution author - Li, Bin; Ricordel, Ivan; Schopfer, Lawrence M; Baud, Frederic; Megarbane, Bruno; Masson, Patrick; Lockridge, Oksana
DOI - 1f40a5db-07bc-4e56-b55dcsaobj201; 14821355; 1099-1263 English

775. Li, Bin; Ricordel, Ivan; Schopfer, Lawrence M; Baud, Frederic; Megarbane, Bruno; Nachon, Florian; Masson, Patrick; Lockridge, Oksana, and Li, Bin. Detection of Adduct on Tyrosine 411 of Albumin in Humans Poisoned by Dichlorvos. 2010 Jul; 116, (1): 23-31.


Rec #: 44069
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: Studies in mice and guinea pigs have shown that albumin is a new biomarker of organophosphorus toxicant (OP) and nerve agent exposure. Our goal was to determine whether OP-labeled albumin could be detected in the blood of humans exposed to OP. Blood from four OP-exposed patients was prepared for mass spectrometry analysis by digesting 0.010 ml of serum with pepsin and purifying the labeled albumin peptide by offline high performance liquid chromatography. Dimethoxyphosphate-labeled tyrosine 411 was identified in albumin peptides VRY411TKKVPQVSTPTL and LVRY411TKKVPQVSTPTL from two patients who had attempted suicide with dichlorvos. The butyrylcholinesterase activity in these serum samples was inhibited 80%. A third patient whose serum BChE activity was inhibited 8% by accidental inhalation of dichlorvos had undetectable levels of adduct on albumin. A fourth patient whose BChE activity was inhibited 60% by exposure to chlorpyrifos had no detectable adduct on albumin. This is the first report to demonstrate the presence of OP-labeled albumin in human patients. It is concluded that tyrosine 411 of human albumin is covalently modified in the serum of humans poisoned by dichlorvos and that the modification is detectable by mass spectrometry. The special reactivity of tyrosine 411 with OP suggests that other proteins may also be modified on tyrosine. Identification of other OP-modified proteins may lead to an understanding of neurotoxic symptoms that appear long after the initial OP exposure.
Keywords: Inhalation
Keywords: High-performance liquid chromatography
Keywords: nerve agents
Keywords: Toxicants
Keywords: Adducts
Keywords: N3 11028:Neuropharmacology & toxicology
Keywords: Toxicology Abstracts; CSA Neurosciences Abstracts
Keywords: Pepsin A
Keywords: Suicide
Keywords: Tyrosine
Keywords: biomarkers
Keywords: Mass spectroscopy
Keywords: Chlorpyrifos
Keywords: Blood
Keywords: Neurotoxicity
Keywords: Albumin
Keywords: X 24330:Agrochemicals
Keywords: Dichlorvos
Date revised - 2010-08-01
Language of summary - English
Pages - 23-31
ProQuest ID - 754534068
SubjectsTermNotLitGenreText - nerve agents; High-performance liquid chromatography; Inhalation; Toxicants; Adducts; Pepsin A; Tyrosine; Suicide; biomarkers; Mass spectroscopy; Chlorpyrifos; Blood; Neurotoxicity; Albumin; Dichlorvos
Last updated - 2012-03-29
British nursing index edition - Toxicological Sciences [Toxicol. Sci.]. Vol. 116, no. 1, pp. 23-31. Jul 2010.
Corporate institution author - Li, Bin; Ricordel, Ivan; Schopfer, Lawrence M; Baud, Frederic; Megarbane, Bruno; Nachon, Florian; Masson, Patrick; Lockridge, Oksana
DOI - 5031d47e-e6c1-4ca2-878acsamfg201; 13237185; 1096-6080 English

776. Li, Bin; Schopfer, Lawrence M; Grigoryan, Hasmik; Thompson, Charles M; Hinrichs, Steven H; Masson, Patrick, and Lockridge, Oksana. Tyrosines of Human and Mouse Transferrin Covalently Labeled by Organophosphorus Agents: a New Motif for Binding to Proteins That Have No Active Site Serine. 2009 Jan; 107, (1): 144-155.


Rec #: 48989
Keywords: IN VITRO
Notes: Chemical of Concern: CPY
Abstract: Abstract: The expectation from the literature is that organophosphorus (OP) agents bind to proteins that have an active site serine. However, transferrin, a protein with no active site serine, was covalently modified in vitro by 0.5mM 10-fluoroethoxyphosphinyl-N-biotinamido pentyldecanamide, chlorpyrifos oxon, diisopropylfluorophosphate, dichlorvos, sarin, and soman. The site of covalent attachment was identified by analyzing tryptic peptides in the mass spectrometer. Tyr 238 and Tyr 574 in human transferrin and Tyr 238, Tyr 319, Tyr 429, Tyr 491, and Tyr 518 in mouse transferrin were labeled by OP. Tyrosine in the small synthetic peptide ArgTyrThrArg made a covalent bond with diisopropylfluorophosphate, chlorpyrifos oxon, and dichlorvos at pH 8.3. These results, together with our previous demonstration that albumin and tubulin bind OP on tyrosine, lead to the conclusion that OP bind covalently to tyrosine, and that OP binding to tyrosine is a new OP-binding residue. The OP-reactive tyrosines are activated by interaction with Arg or Lys. It is suggested that many proteins in addition to those already identified may be modified by OP on tyrosine. The extent to which tyrosine modification by OP can occur in vivo and the toxicological implications of such modifications require further investigation.
Keywords: Animals
Keywords: 96-64-0
Keywords: Biotin -- analogs & derivatives
Keywords: Soman -- metabolism
Keywords: Humans
Keywords: Tyrosine
Keywords: Organophosphorus Compounds
Keywords: Organophosphorus Compounds -- metabolism
Keywords: Transferrin -- physiology
Keywords: Tyrosine -- metabolism
Keywords: Biotin
Keywords: Biotin -- metabolism
Keywords: 55520-40-6
Keywords: Mice
Keywords: 58-85-5
Keywords: Tandem Mass Spectrometry
Keywords: Binding Sites
Keywords: Transferrin -- metabolism
Keywords: Blotting, Western
Keywords: Transferrin
Keywords: 0
Keywords: Soman
Keywords: Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Keywords: Models, Chemical
Keywords: 10-(fluoroethoxyphosphinyl)-N-(biotinamidopentyl)decanamide
Date completed - 2009-08-07
Date created - 2008-12-16
Date revised - 2012-12-20
Language of summary - English
Pages - 144-155
ProQuest ID - 66739943
SuppNotes - Cites: J Biol Chem. 1973 May 10;248(9):3228-32[4735577]; Cites: Chem Res Toxicol. 2008 Sep;21(9):1787-94[18707141]; Cites: Proc Natl Acad Sci U S A. 1982 Apr;79(8):2504-8[6953407]; Cites: Toxicol Appl Pharmacol. 1997 Oct;146(2):227-36[9344890]; Cites: Electrophoresis. 1999 Dec;20(18):3551-67[10612281]; Cites: Toxicol Lett. 2001 Feb 3;119(1):21-6[11275418]; Cites: J Appl Toxicol. 2001 Dec;21 Suppl 1:S103-7[11920929]; Cites: Toxicol Appl Pharmacol. 2002 Jul 15;182(2):176-85[12140181]; Cites: Toxicology. 2003 May 3;187(2-3):195-203[12699908]; Cites: Biochem J. 1957 Jun;66(2):237-42[13445678]; Cites: Arch Toxicol. 2004 Sep;78(9):508-24[15170525]; Cites: Toxicol Sci. 2004 Dec;82(2):545-54[15342957]; Cites: Toxicol Sci. 2005 Feb;83(2):303-12[15525694]; Cites: Toxicol Sci. 2005 Aug;86(2):291-9[15888665]; Cites: Biochem Pharmacol. 2005 Nov 25;70(11):1673-84[16213467]; Cites: J Biol Chem. 2006 Aug 25;281(34):24934-44[16793765]; Cites: Toxicol Appl Pharmacol. 2007 Jan 1;218(1):20-9[17123561]; Cites: Neuroscience. 2007 Apr 25;146(1):330-9[17321052]; Cites: J Pharmacol Exp Ther. 2007 Sep;322(3):1117-28[17548533]; Cites: Chem Biol Interact. 2008 Sep 25;175(1-3):180-6[18502412]; Cites: J Biol Chem. 2008 Aug 15;283(33):22582-90[18577514]; Cites: Comp Biochem Physiol B. 1989;93(2):417-24[2776434]; Cites: J Biol Chem. 1969 Aug 10;244(15):4247-50[5800444]; Cites: Toxicol Appl Pharmacol. 1997 Jul;145(1):158-74[9221834]; Cites: J Toxicol Environ Health B Crit Rev. 1999 Apr-Jun;2(2):161-81[10230392]; Cites: Mol Pharmacol. 2000 Sep;58(3):577-83[10953051]; Cites: Chem Res Toxicol. 2002 Apr;15(4):582-90[11952345]; Cites: Toxicol Lett. 2002 Sep 5;135(1-2):89-93[12243867]; Cites: Environ Health Perspect. 2003 Nov;111(14):1736-43[14594624]; Cites: Chem Res Toxicol. 2004 Aug;17(8):983-98[15310231]; Cites: Toxicol Sci. 2005 Jan;83(1):166-76[15470232]; Cites: Anal Biochem. 2005 Oct 1;345(1):122-32[16125664]; Cites: Curr Med Chem. 2005;12(23):2683-93[16305465]; Cites: Anal Biochem. 2007 Feb 15;361(2):263-72[17188226]; Cites: Arch Toxicol. 2007 Sep;81(9):627-39[17345062]; Cites: Chem Res Toxicol. 2008 Feb;21(2):421-31[18163544]; Cites: Biochemistry. 1965 Dec;4(12):2815-25[5880690]
Last updated - 2013-01-19
British nursing index edition - Toxicological sciences : an official journal of the Society of Toxicology, January 2009, 107(1):144-155
Corporate institution author - Li, Bin; Schopfer, Lawrence M; Grigoryan, Hasmik; Thompson, Charles M; Hinrichs, Steven H; Masson, Patrick; Lockridge, Oksana
DOI - MEDL-18930948; 18930948; PMC2638647; 1096-0929 eng

777. Li, H. B.; Li, J.; Xu, Q., and Hu, X. Y. Poly(3-hexylthiophene)/TiO(2) Nanoparticle-Functionalized Electrodes for Visible Light and Low Potential Photoelectrochemical Sensing of Organophosphorus Pesticide Chlopyrifos. 2011; 83, (24): 9681-9686.


Rec #: 63829
Keywords: CHEM METHODS
Notes: Chemical of Concern: CPY
Abstract: Abstract: A dramatic visible light photoelectrochemical sensing platform for the detection of pesticide molecules at zero potential (versus saturated calomel electrode) was first constructed using poly(3-hexylthiophene)-functionalized TiO(2) nanoparticles. Poly(3-hexylthiophene) (P3HT) was synthesized via chemical oxidative polymerization with anhydrous FeCl(3) as the oxidant, 3-hexylthiophene as the monomer, and chloroform as the solvent, and the functional TiO(2) nanoparticles were facilely prepared by blending TiO(2) nanoparticles and P3HT in chloroform solution. The resulting photoelectrocatalysts were characterized by scanning electron microscopy, Raman spectroscopy, and X-ray diffractometry. Under visible light irradiation, P3HT generated the transition from the valence band to the conduction band, delivering the excited electrons into the conduction band of TiO(2) and then to the glassy carbon electrode. Simultaneously, a positive charged hole (h(+)) of TiO(2) may form and migrate to the valence band of P3HT, which can react with H(2)O to generate *OH, and then it converted chlopyrifos into chlopyrifos* that promoted the amplifying photocurrent response. On the basis of the proposed photoelectrochemical mechanism, a methodology for sensitive photoelectrochemical sensing for chlopyrifos at zero potential was thus developed. Under optimal conditions, the proposed photoelectrochemical method could detect chlopyrifos ranging from 0.2 to 16 mu mol L(-1) with a detection limit of 0.01 mu mol L(-1) at a signal-to-noise ratio of 3. The photoelectrochemical sensor had an excellent specificity against the other pesticides and could be successfully applied to the detection of reduced chlopyrifos in green vegetables, showing a promising application in photoelectrochemical sensing.
Keywords: SOLAR-CELLS, PHOTOCATALYTIC ACTIVITY, GAS-CHROMATOGRAPHY,
ISI Document Delivery No.: 860KJ

778. Li, Huizhen ; Tyler Mehler, W; Lydy, Michael J; You, Jing, and Li, Huizhen. Occurrence and Distribution of Sediment-Associated Insecticides in Urban Waterways in the Pearl River Delta, China. 2011 Mar; 82, (10): 1373-1379.


Rec #: 43509
Keywords: FATE
Notes: Chemical of Concern: CPY
Abstract: Abstract: Sediment-associated pesticides, including organochlorine (OCP), organophosphate (OP), and pyrethroid insecticides, were analyzed in urban waterways in three cities (Guangzhou, Dongguan, and Shenzhen) in the Pearl River Delta (PRD), China. The OCPs represented 27.2% of the detectable insecticides in sediment, and chlordanes, DDTs, and endosulfans were the most frequently detected OCPs. The currently used insecticide chlorpyrifos was the only OP detected above the reporting limit (RL), with concentrations ranging from Keywords: Organochlorine compounds
Keywords: China, People's Rep., Zhu R.
Keywords: ISEW, China, People's Rep., Guangdong Prov., Shenzhen
Keywords: Urbanization
Keywords: Organophosphates
Keywords: Formicidae
Keywords: Q2 02264:Sediments and sedimentation
Keywords: Deltas
Keywords: Q5 01502:Methods and instruments
Keywords: Freshwater
Keywords: Environmental Studies
Keywords: Insecticides
Keywords: deltas
Keywords: Landscaping
Keywords: Pyrethroids
Keywords: Aquatic insects
Keywords: Rivers
Keywords: Sediment pollution
Keywords: Cypermethrin
Keywords: P 2000:FRESHWATER POLLUTION
Keywords: Chlordane
Keywords: Z 05350:Medical, Veterinary, and Agricultural Entomology
Keywords: Brackish
Keywords: Entomology Abstracts; ASFA 2: Ocean Technology Policy & Non-Living Resources; Environment Abstracts; Pollution Abstracts; Oceanic Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality
Keywords: Pest control
Keywords: organophosphates
Keywords: China, People's Rep., Guangdong Prov., Guangzhou
Keywords: Sediments
Keywords: Maintenance
Keywords: Endosulfan
Keywords: ENA 06:Food & Drugs
Keywords: Chlorpyrifos
Keywords: cypermethrin
Keywords: O 4080:Pollution - Control and Prevention
Keywords: Pesticides
Keywords: DDT
Date revised - 2011-10-01
Language of summary - English
Location - ISEW, China, People's Rep., Guangdong Prov., Shenzhen; China, People's Rep., Zhu R.; China, People's Rep., Guangdong Prov., Guangzhou
Pages - 1373-1379
ProQuest ID - 855539056
SubjectsTermNotLitGenreText - Rivers; Sediment pollution; Insecticides; Urbanization; DDT; Pesticides; Pest control; Deltas; Aquatic insects; Organochlorine compounds; Cypermethrin; Chlordane; organophosphates; Sediments; Endosulfan; Chlorpyrifos; Landscaping; Pyrethroids; cypermethrin; Organophosphates; deltas; Maintenance; Formicidae; ISEW, China, People's Rep., Guangdong Prov., Shenzhen; China, People's Rep., Zhu R.; China, People's Rep., Guangdong Prov., Guangzhou; Brackish; Freshwater
Last updated - 2011-12-17
Corporate institution author - Li, Huizhen; Tyler Mehler, W; Lydy, Michael J; You, Jing
DOI - OB-5363707c-00f6-47f2-a71ccsamfg201; 14366494; CS1130210; 0045-6535 English

779. Li, J. Q.; Liu, J. A.; Shen, W. J.; Zhao, X. L.; Hou, Y.; Cao, H., and Cui, Z. L. Isolation and characterization of 3,5,6-trichloro-2-pyridinol-degrading Ralstonia sp strain T6. 2010; 101, (19): 7479-7483.


Rec #: 63869
Keywords: BACTERIA
Notes: Chemical of Concern: CPY
Abstract: Abstract: A 3,5,6-trichloro-2-pyridinol (TCP)-degrading strain, T6, was isolated by continuous enrichment culture and identified as Ralstonia sp. based on morphological, physiological and biochemical tests as well as 16S rDNA sequence analysis. The bacterium metabolized 100 mg/L TCP within 12 h and 700 mg/L TCP in 80 h. A green metabolite, putatively identified as 3,6-dihydroxypyridine-2,5-dione, was detected. This is the first report of TCP-degrading isolate from the genus of Ralstonia. Strain T6 could potentially be employed in bioremediation of TCP. (C) 2010 Elsevier Ltd. All rights reserved.
Keywords: 3,5,6-Trichloro-2-pyridinol, Biodegradation, Green metabolite, Ralstonia
ISI Document Delivery No.: 625KP

780. Li, K.; Wang, S. H.; Shi, Y. H.; Qu, J.; Zhai, Y.; Xu, L. L. ; Xu, Y. X.; Song, J. L.; Liu, L. L.; Rahman, M. A., and Yan, Y. C. Genome Sequence of Paracoccus sp Strain TRP, a Chlorpyrifos Biodegrader. 2011; 193, (7): 1786-1787.


Rec #: 63879
Keywords: BACTERIA
Notes: Chemical of Concern: CPY
Abstract: Abstract: Paracoccus sp. strain TRP, isolated from activated sludge, could completely biodegrade chlorpyrifos and 3,5,6-trichloro-2-pyridinol. Here we report the draft genome sequence of Paracoccus sp. strain TRP, which could be used to predict genes for xenobiotic biodegradation and metabolism.
Keywords: GENES
ISI Document Delivery No.: 734DW

781. Li, L.; Cao, Z. H.; Jia, P. F., and Wang, Z. R. Calcium signals and caspase-12 participated in paraoxon-induced apoptosis in EL4 cells. 2010; 24, (3): 728-736.


Rec #: 63889
Keywords: IN VITRO
Notes: Chemical of Concern: CPY
Abstract: Abstract: In order to investigate whether calcium signals participate in paraoxon (POX)-induced apoptosis in EL4 cells, real-time laser scanning confocal microscopy (LSCM) was used to detect Ca(2+) changes during the POX application. Apoptotic rates of EL4 cells and caspase-12 expression were also evaluated. POX (1-0 nM) increased intracellular calcium concentration ([Ca(2+)]i) in EL4 cells in a dose-dependent manner at early stage (0-2 h) of POX application, and apoptotic rates of EL4 cells after treatment with POX for 16 h were also increased in a dose-dependent manner. Pre-treatment with EGTA, heparin or procaine attenuated POX-induced [Ca(2+)]i elevation and apoptosis. Additionally, POX up-regulated caspase-12 expression in a dose-dependent manner, and pre-treatment with EGTA, heparin or procaine significantly inhibited POX-induced increase of caspase-12 expression. Our results suggested that POX induced [Ca(2+)]i elevation in EL4 cells at the early stage of POX-induced apoptosis, which might involve Ca(2+) efflux from the endoplasmic reticulum (ER) and Ca(2+) influx from extracellular medium. Calcium signals and caspase-12 were important upstream messengers in POX-induced apoptosis in EL4 cells. The ER-associated pathway possibly operated in this apoptosis. (C) 2010 Elsevier Ltd. All rights reserved.
Keywords: Paraoxon, EL4 cells, Apoptosis, Calcium signals, Endoplasmic reticulum,
ISI Document Delivery No.: 583IT

782. Li, Q; Kobayashi, M; Kawada, T, and Li, Q. Chlorpyrifos Induces Apoptosis in Human T Cells. 2009 Jan 8; 255, (1-2): 53-57.


Rec #: 41479
Keywords: HUMAN HEALTH
Notes: Chemical of Concern: CPY
Abstract: Abstract: It was found previously that organophosphorus pesticides (OPs) significantly inhibited cytotoxic T lymphocyte (CTL) activity. To explore the mechanism of OP-induced inhibition of CTL activity, the present study investigated whether OPs can induce cell death /apoptosis in T cells. Jurkat human T cells were treated with chlorpyrifos at 0-100ppm for 2, 4, and 6h at 37 super(o)C in vitro. It was found that chlorpyrifos induced cell death of Jurkat human T cells in a dose- and time-dependent manner, as shown by MTT and LDH assays. Then, it was investigated if chlorpyrifos-induced cell death consisted of apoptosis, as determined by analysis of Annexin-V staining and the intracellular level of active caspase-3 by flow cytometry, and DNA fragmentation analysis. It was found that chlorpyrifos induces apoptosis in Jurkat T cells in a dose- and time-dependent manner, as determined by analysis of Annexin-V staining. DNA fragmentation was detected when cells were treated with 50 or 100ppm chlorpyrifos for 4 and 6h. Chlorpyrifos also induced an increase in intracellular active caspase-3 in Jurkat T cells in a dose- and time-dependent manner, and a caspase-3 inhibitor, Z-DEVD-FMK, significantly inhibited chlorpyrifos-induced apoptosis. These findings indicate that chlorpyrifos can induce apoptosis in human Jurkat T cell cells, and this effect is partially mediated by the activation of intracellular caspase-3.
Keywords: Pesticides (organophosphorus)
Keywords: Pharmacy And Pharmacology
Keywords: Apoptosis
Keywords: F 06955:Immunomodulation & Immunopharmacology
Keywords: Intracellular levels
Keywords: Toxicology Abstracts; Immunology Abstracts
Keywords: Cell activation
Keywords: Chlorpyrifos
Keywords: Flow cytometry
Keywords: DNA fragmentation
Keywords: Cytotoxicity
Keywords: Lymphocytes T
Keywords: Caspase-3
Keywords: X 24330:Agrochemicals
Date revised - 2011-05-01
Language of summary - English
Pages - 53-57
ProQuest ID - 294613496
SubjectsTermNotLitGenreText - Flow cytometry; Chlorpyrifos; Pesticides (organophosphorus); DNA fragmentation; Cytotoxicity; Apoptosis; Intracellular levels; Caspase-3; Lymphocytes T; Cell activation
Last updated - 2011-11-10
Corporate institution author - Kobayashi, M; Kawada, T
DOI - OB-MD-0009064538; 8869193; 0300-483X English

783. Li, S.; Liu, Y.; Ni, Z.; Song, X.; Liu, X., and Zhao, L. Mutagenicity of 24 Organophosphorus Pesticides Determined by 6 Short-term Tests. S.Li, Res. Lab. Toxicol., West China Univ. Med. Sci., Chengdu, 610041, Peop. Rep. China//: 1993; 7, (1): 73-77(CHI).


Rec #: 1050
Keywords: NOT PURSUING,NON-ENGLISH
Call Number: NON-ENGLISH (CPY)
Notes: Chemical of Concern: CPY

784. Li, T. G.; Nana, W.; Heng, D., and Min, Z. Polyethylene glycosylation prolongs the stability of recombinant human paraoxonase-1. 2012; 210, (3): 366-371.


Rec #: 63949
Keywords: IN VITRO
Notes: Chemical of Concern: CPY
Abstract: Abstract: Paraoxonase-1 (PON1) is a native enzyme that is synthesized in the liver and is capable of hydrolyzing organophosphates (OPs). It is regarded as part of a promising approach for the pretreatment and therapy of OP poisoning. Previous experiments with purified rabbit serum PON1 have established that
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