Appendix 2-5: Rejected ecotox bibliography for Chlorpyrifos

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cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJ1C assay. The concentration-dependent inhibition of GJ1C was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJlC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity. (c) 2007 Elsevier Ltd. All rights reserved.
Keywords: diazinon, parathion, methyl-parathion, ozone, buffalo rat liver
ISI Document Delivery No.: 212LS

1525. Wu, N.; Hao, F. T., and Yu, X. J. Peripheral nerve and skin damage associated with working in a STCP factory: Report of four cases. 2012; 50, (6): 514-517.

Rec #: 72179
Notes: Chemical of Concern: CPY
Abstract: Abstract: Sodium 3,5,6-trichloropyridin-2-ol (STCP) is a widely used intermediate for the production of chlorpyrifos. However, its effect on the health of workers in STCP factories has become increasingly problematic. This article reports four workers who worked in a STCP factory developed peripheral neuropathy in the lower extremities and chloracne-like skin lesions on the whole body. Patches of follicular skin eruptions (mainly blackheads, accompanied by follicular orifice cornification occasionally accompanied by milia-like skin eruptions) were found on the head and face, around the auricle, chest and back, abdomen and scrotum. In one patient, the activity of serum cholinesterase was decreased and recovered slowly, but there were no muscarinic, nicotinic, or central nervous system symptoms. The concentrations of urine STCP detected in four patients were 0.266, 0.066, 0.044, and 0.033 mu g/mL.
Keywords: Sodium 3,5,6-trichloropyridin-2-ol (STCP), Chloracne, Cholinesterase
ISI Document Delivery No.: 966LI

1526. Wu, W. M.; Wu, Y. M.; Zheng, M. M.; Yang, L. M.; Wu, X. P.; Lin, X. C., and Xie, Z. H. Pressurized capillary electrochromatography with indirect amperometric detection for analysis of organophosphorus pesticide residues. 2010; 135, (8): 2150-2156.

Rec #: 72199
Notes: Chemical of Concern: CPY
Abstract: Abstract: A new analytical method, pressurized capillary electrochromatography with indirect amperometric detection, has been developed for the determination of some non-electroactive organophosphorus pesticides (OPPs). When 0.1 mmol L(-1) of 3,4-dihydroxybenzylamine (DHBA) was added to the mobile phase containing 50% v/v of ACN and 50% v/v of MES buffer (10 mmol L(-1), pH 5.5), and +0.9 V (vs. Ag/AgCl) of working potential were used, maximal signal levels of analytes could be achieved. A separation voltage of + 10 kV, a column pressure of 7.0 MPa and a pump flow rate of 0.05 mL min(-1) were selected as the other optimal conditions for separation of six OPPs, namely, dimethoate, methyl parathion, ethyl parathion, chlorpyrifos, chlorpyrifos-methyl, trichlorfon. The OPPs could be separated within 15 min and determined with the detection limits ranging from 0.008 to 0.2 mg/kg. Combining with a solid phase extraction procedure, mean recoveries between 78.9 and 87.2% for vegetable samples and from 81.4 to 98.6% for fruit samples were obtained.
ISI Document Delivery No.: 628AR

1527. Wu, X. M.; Bennett, D. H.; Ritz, B.; Frost, J.; Cassady, D.; Lee, K., and Hertz-Picciotto, I. Residential insecticide usage in northern California homes with young children. 2011; 21, (4): 427-436.

Rec #: 72209
Notes: Chemical of Concern: CPY
Abstract: Abstract: Residential insecticide usage and actual application details were collected in a population-based sample of 477 households residing within 22 counties in northern California with at least one child of age <= 5 years between January 2006 and August 2008. Structured telephone interviews were conducted collecting information on residential use of insecticides, including outdoor sprays, indoor sprays, indoor foggers, applications by professionals, and pet flea/tick control during the previous year. Interviews also covered post-treatment behaviors, which influence post-application exposure levels. Altogether, 80% of the households applied some type of insecticide in the previous year, with half of this population using two or more application methods. Of the households using insecticides, half reported applying insecticides relatively infrequently (<4 times per year), whereas 11-13% reported high frequency of use ( 424 times per year). Application frequency was temperature dependent, with significantly more applications during the warmer months from May through October. Spot treatments appeared to be the most prevalent application pattern for sprays. For one out of three of the indoor applications, children played in the treated rooms on the day of the application, and for 40% of the outdoor applications, pets played in the treated area on the day of the application. These findings describing the intensity of insecticide use and accompanying behaviors in families with young children may inform future insecticide exposure modeling efforts, and ultimately, risk assessments. Journal of Exposure Science and Environmental Epidemiology (2011) 21, 427-436; doi: 10.1038/jes.2010.36; published online 30 June 2010
Keywords: insecticide, residential exposure, exposure-related behavior,
ISI Document Delivery No.: 780LT

1528. Wu, X. W.; Cheng, L. Y.; Cao, Z. Y., and Yu, Y. L. Accumulation of chlorothalonil successively applied to soil and its effect on microbial activity in soil. 2012; 81, 65-69.

Rec #: 72219
Keywords: BACTERIA
Notes: Chemical of Concern: CPY
Abstract: Abstract: The effect of successive chlorothalonil applications on the persistence of chlorothalonil, soil respiration activity, and dehydrogenase activity (DHA) in soil was investigated under laboratory conditions. The persistence of chlorothalonil in soil was prolonged significantly with the increase in the concentration applied. Repeated applications of chlorothalonil at 25 mg kg(-1) led to its accumulation in soil. The effect of repeated chlorothalonil applications on soil respiration and DHA was found to be a concentration-dependent process. Soil respiration was permanently inhibited by the successive introductions of chlorothalonil at 25 mg kg(-1). DHA was reduced significantly on day 15 after four successive treatments of 10 mg kg(-1) and 25 mg kg(-1) of chlorothalonil, although a recovery trend could be found after the third and fourth treatments. Repeated chlorothalonil applications might increase the persistence of chlorothalonil in soil and thus alter soil microbial activity. (C) 2012 Elsevier Inc. All rights reserved.
Keywords: Chlorothalonil, Microbial activity, Accumulation, Repeated applications
ISI Document Delivery No.: 966MV

1529. Wu, X-W; Hua, R-M; Tang, J, and Wu, X-W. Effect of Surfactants on Photolysis of Chlorpyrifos in Aqueous Solution. 2009 Aug; 28, (8): 1705-1711.

Rec #: 41089
Keywords: FATE
Notes: Chemical of Concern: CPY
Abstract: Abstract: The effect of different surfactants on photolysis of chlorpyrifos was investigated in aqueous solutions under UV and high pressure mercury lamp(HPML) irradiation. The results indicated that photodegradation all well followed pseudo-first-order kinetics under various conditions. No photosensitive effect was observed in the solutions with low dosage SDBS under UV radiation, and its photosensitive efficiency against chlorpyrifos was 11.61% at the concentration of 50 mg times L super(-1) SDBS. The photosensitive efficiency of chlorpyrifos in the presence of 1, 10 and 50 mg times L super(-1) Tween80 was 7%, 24.79% and 90.20%, respectively. When concentration of Span20 was 10, 50 mg times L super(-1), its photosensitive efficiency was 33.07%, 65.49%, respectively. The half lives of chlorpyrifos were found to be 1.32 h, 1.29 h for aqueous solutions containing 50 mg times L super(-1) 0201 and 2201, respectively. Under HPML irradiation, the photolysis of chlorpyrifos was accelerated by Nongru404, Nongru603, Nongru500, Nongru601 and 0206B. The photolysis rate of chlorpyrifos was 1.73 and 2.22 times greater in the presence of 25 mg times L super(-1) Nongru404 and Nongru601 surfactants than that in water alone, respectively. However, little photosensitive efficiency was found on photolysis of chlorpyrifos in the presence of Nongru500, 0206B and Nongru603. An increased absorption of chlorpyrifos solutions in ultraviolet part of the spectrum was responsible for photosensitive efficiency. The results also indicated that the photosensitive efficiency of chlorpyrifos was related to types and dosage of surfactants.
Keywords: Photolysis
Keywords: Chlorpyrifos
Keywords: Efficiency
Keywords: Photodegradation
Keywords: P 8000:RADIATION
Keywords: Irradiation
Keywords: Kinetics
Keywords: Pesticides
Keywords: Ultraviolet radiation
Keywords: Absorption
Keywords: Mercury
Keywords: Pollution Abstracts
Keywords: Surfactants
Date revised - 2009-10-01
Language of summary - English
Pages - 1705-1711
ProQuest ID - 20825923
SubjectsTermNotLitGenreText - Chlorpyrifos; Photolysis; Efficiency; Photodegradation; Kinetics; Irradiation; Ultraviolet radiation; Pesticides; Absorption; Mercury; Surfactants
Last updated - 2011-12-14
British nursing index edition - Journal of Agro-Environment Science [J. Agro-Environ. Sci.]. Vol. 28, no. 8, pp. 1705-1711. Aug 2009.
Corporate institution author - Wu, X-W; Hua, R-M; Tang, J
DOI - MD-0010549247; 10982776; 1672-2043 English

1530. Xia, X. J. ; Zhang, Y.; Wu, J. X.; Wang, J. T.; Zhou, Y. H.; Shi, K.; Yu, Y. L., and Yu, J. Q. Brassinosteroids Promote Metabolism of Pesticides in Cucumber. 2009; 57, (18): 8406-8413.

Rec #: 72229
Keywords: FOOD
Notes: Chemical of Concern: CPY
Abstract: Abstract: Brassinosteroids (BRs) are known to protect crops from the toxicity of herbicides, fungicides and insecticides, It is shown here that application of 24-epibrassinolide (EBR) accelerated metabolism of various pesticides and conse uently reduced their residual levels in cucumber (Cucumis sativus L). Chlorpyrifos, a widely used insecticide, caused significant reductions of net photosynthetic rate (Pn) and quantum yield of PSII (Phi(PSII) in cucumber leaves. EBR pretreatment alleviated the declines of Pn and Phi(PSII) caused by chlorpyrifos application, and this effect of EBR was associated with reductions of chlorpyrifos residues. o understand how EBR promotes chlorpyrifos metabolism, the effects of EBR on activity and of en ymes involved in pesticide metabolism were analyed. EBR had a positive effect on the activation of glutathione S-transferase (GST), peroxclase (POD), and glutathione reductase (GR) after treatment with chlorpyrifos, although the effect on GR was attenuated at later time points when plants were treated with 1 mM chlorpyrifos. In addition, EBR enhanced the epression of P450 and MRP, which encode P450 monooxygenase and ABCtype transporter, respectively. owever, the epression of GST was consistently lower than that of plants treated with only chlorpyrifos. Importantly, the stimulatory effect of EBR on pesticide metabolism was also observed for cypermethrin, chlorothalonil, and carbenda im, which was attributed to the enhanced activity and genes involved in pesticide metabolism. he results suggest that BRs may be promising, environmentally friendly, natural substances suitable for wide application to reduce the ris s of human and environment e posure tc pesticides.
Keywords: Brassinosteroids, Cucumis sativus, degradation, glutathione, enzyme
ISI Document Delivery No.: 493OS

1531. Xie, C. G.; Li, H. F.; Li, S. Q.; Wu, J., and Zhang, Z. P. Surface Molecular Self-Assembly for Organophosphate Pesticide Imprinting in Electropolymerized Poly(p-aminothiophenol) Membranes on a Gold Nanoparticle Modified Glassy Carbon Electrode. 2010; 82, (1): 241-249.

Rec #: 72239
Notes: Chemical of Concern: CPY
Abstract: Abstract: This paper reports a surface molecular self-assembly strategy for molecular imprinting in electropolymerized polyaminothiophenol (PATP) membranes at the surface of gold nanoparticles (AuNPs) modified glassy carbon (gc) electrode for the electrochemical detection of pesticide chlorpyrifos (CPF). Electropolmerizable p-aminothiophenol (ATP) was first assembled on the AuNPs at the gc electrode surface by the formation of Au-S bonds, and subsequently, the CPF template was further assembled onto the monolayer of ATP through the hydrogen-bonding interaction between amino group and CPF. A conductive hybrid membrane was fabricated at the assembled gc electrode by the electropolymerization in a mixing solution containing additional ATP and CPF templates, and meanwhile, the CPF was spontaneously, imprinted into the PATP/AuNP film. The amount of imprinted sites at the PATP/AuNP film was significantly increased due to the additional replenishment of CPF templates. The cyclic voltammetric response of the imprinted PATP-AuN-P-gc sensor to CPF is about 3.2-fold as compared with the imprinted PATP-Au sensor, and the detection limit for CPF is about 2 orders of magnitude lower than that by the imprinted PATP-Au sensor. An excellent electrochemical selectivity for CPF over other pesticides was also achieved. The combination of surface molecular self-assembly with electropolymerized molecular imprinting on a larger surface area of a AuNP-modified electrode produces a high ratio of imprinted sites and, thus, provides an ultrasensitive electrochemical detection of organophosphate pesticide.
ISI Document Delivery No.: 539OT

1532. Xie, H; Zhu, L; Wang, J, and Xie, H. Determination of Chlorpyrifos Residues in Water, Cabbage and Soil by Gc-Fpd. 2012 Aug; 31, (8): 1268-1274.

Rec #: 38619
Keywords: SURVEY
Notes: Chemical of Concern: CPY
Abstract: Abstract: Using gas chromatography-flame photometric detection (GC-FPD), a method was developed for the analysis of organophosphorus pesticide chlorpyrifos in water, soil and cabbage. The chlorpyrifos residue in water was extracted and purified by petroleum ether and liquid-liquid extraction. The chlorpyrifos residue in cabbage was extracted with acetone and the chlorpyrifos residue in soil was extracted by the Soxhlet method with acetone. After clean up by liquid-liquid distribution, it was determinated by GC-FPD equipped with OV-101(100% dimethyl polysiloxane) column (30 m x 0.53 mm x 1.0 mu m). The retention time of chlorpyrifos was 1.74 min; the linearity range of chlorpyrifos was between 1.0 x 10 super(-11) and 1.0 x 10 super(-8) g; the correlation coefficient was 0.9998; the limits of detection was 2.0 x 10 super(-12) g. Average recovery rates of chlorpyrifos in these samples ranged from 80% to 120% with coefficient of variation from 1.19% to 4.40%. The method is sensitive and operated easily to detect low levels of chlorpyrifos in the water, cabbage and soil.
Keywords: Residues
Keywords: ENA 09:Land Use & Planning
Keywords: Meteorological & Geoastrophysical Abstracts; Environment Abstracts
Keywords: Correlations
Keywords: Brassica
Keywords: Chlorpyrifos
Keywords: Soil
Keywords: Petroleum
Keywords: Photometric observations
Keywords: Pesticides
Keywords: M2 551.5:General (551.5)
Keywords: Acetone
Keywords: Ethers
Date revised - 2012-10-01
Language of summary - English
Pages - 1268-1274
ProQuest ID - 1125239160
SubjectsTermNotLitGenreText - Photometric observations; Correlations; Soil; Chlorpyrifos; Residues; Petroleum; Pesticides; Ethers; Acetone; Brassica
Last updated - 2012-12-03
British nursing index edition - Environmental Chemistry - Huanjing Huaxue. Vol. 31, no. 8, pp. 1268-1274. Aug 2012.
Corporate institution author - Xie, H; Zhu, L; Wang, J
DOI - MD-0019988760; 17299975; 0254-6108 English

1533. Xie, Hui; Zhu, Lusheng; Ma, Tingting; Wang, Jun; Wang, Jinhua; Su, Jun; Shao, Bo, and Xie, Hui. Immobilization of an Enzyme From a Fusarium Fungus Wz-I for Chlorpyrifos Degradation. 2010 Dec; 22, (12): 1930-1935.

Rec #: 40199
Keywords: FATE
Notes: Chemical of Concern: CPY
Abstract: Abstract: The free enzyme extracted from WZ-I, which was identified as Fusarium LK. ex Fx, could effectively degrade chlorpyrifos, an organophosphate insecticide. The methods of immobilizing this free enzyme and determined its degradation-related characteristics were investigated. The properties of the immobilized enzyme were compared with those of the free enzyme. The optimal immobilization of the enzyme was achieved in a solution of 30 g/L sodium alginate at 4[deg]C for 4-12 hr. The immobilized enzyme showed the maximal activity at pH 8.0, 45[deg]C. The maximum initial rate and the substrate concentration of the immobilized enzyme were less than that of the free enzyme. The immobilized enzyme, therefore, had a higher capacity to withstand a broader range of temperatures and pH conditions than the free enzyme. With varying pH and temperatures, the immobilized enzyme was more active than the free enzyme in the degradation reaction. In addition, the immobilized enzyme exhibited only a slight loss in its initial activity, even after three repeated uses. The results showed that the immobilized enzyme was more resistant to different environmental conditions, suggesting that it was viable for future practical use.
Keywords: Fusarium
Keywords: Degradation
Keywords: Organophosphates
Keywords: Temperature
Keywords: Enzymes
Keywords: Environmental Studies
Keywords: Chlorpyrifos
Keywords: Sodium
Keywords: Insecticides
Keywords: Pesticides
Keywords: Environment Abstracts
Keywords: ENA 21:Wildlife
Keywords: pH
Date revised - 2011-06-01
Language of summary - English
Pages - 1930-1935
ProQuest ID - 886113020
SubjectsTermNotLitGenreText - Sodium; Chlorpyrifos; Insecticides; Degradation; Organophosphates; Pesticides; Temperature; Enzymes; pH; Fusarium
Last updated - 2011-10-24
Corporate institution author - Xie, Hui; Zhu, Lusheng; Ma, Tingting; Wang, Jun; Wang, Jinhua; Su, Jun; Shao, Bo
DOI - OB-79166c38-6288-4fc1-b60acsamfg201; 14443019; 1001-0742 English

1534. Xie, Shaofei; Xiang, Bingren; Yu, Liyan, and Deng, Haishan. Tailoring Noise Frequency Spectrum to Improve Nir Determinations. 2009 Dec 15; 80, (2): 895-902.

Rec #: 44429
Notes: Chemical of Concern: CPY
Abstract: Abstract: Near infrared spectroscopy (NIR) contains excessive background noise and weak analytical signals caused by near infrared overtones and combinations. That makes it difficult to achieve quantitative determinations of low concentration samples by NIR. A simple chemometric approach has been established to modify the noise frequency spectrum to improve NIR determinations. The proposed method is to multiply one Savitzky-Golay filtered NIR spectrum with another reference spectrum added with thermal noises before the other Savitzky-Golay filter. Since Savitzky-Golay filter is a kind of low-pass filter and cannot eliminate low frequency components of NIR spectrum, using one step or two consecutive Savitzky-Golay filter procedures cannot improve the determination of NIR greatly. Meanwhile, significant improvement is achieved via the Savitzky-Golay filtered NIR spectrum processed with the multiplication alteration before the other Savitzky-Golay filter. The frequency range of the modified noise spectrum shifts toward higher frequency regime via multiplication operation. So the second Savitzky-Golay filter is able to provide better filtering efficiency to obtain satisfied result. The improvement of NIR determination with tailoring noise frequency spectrum technique was demonstrated by both simulated dataset and two measured NIR spectral datasets. It is expected that noise frequency spectrum technique will be adopted mostly in applications where quantitative determination of low concentration sample is crucial.
Keywords: 2921-88-2
Keywords: Antiviral Agents -- administration & dosage
Keywords: Water Pollutants, Chemical -- analysis
Keywords: Humans
Keywords: Algorithms
Keywords: Chromatography, High Pressure Liquid
Keywords: Antiviral Agents -- blood
Keywords: Chlorpyrifos -- analysis
Keywords: Water Supply -- analysis
Keywords: Insecticides
Keywords: Water Pollutants, Chemical
Keywords: Male
Keywords: 59277-89-3
Keywords: Capsules
Keywords: Signal Processing, Computer-Assisted
Keywords: Reproducibility of Results
Keywords: Acyclovir -- administration & dosage
Keywords: Antiviral Agents
Keywords: Insecticides -- analysis
Keywords: Chlorpyrifos
Keywords: Acyclovir -- blood
Keywords: Acyclovir
Keywords: 0
Keywords: Spectroscopy, Near-Infrared -- methods
Keywords: Spectroscopy, Near-Infrared -- instrumentation
Date completed - 2010-01-20
Date created - 2009-10-19
Date revised - 2012-12-20
Language of summary - English
Pages - 895-902
ProQuest ID - 734093858
Last updated - 2013-01-19
British nursing index edition - Talanta, December 15, 2009, 80(2):895-902
Corporate institution author - Xie, Shaofei; Xiang, Bingren; Yu, Liyan; Deng, Haishan
DOI - MEDL-19836570; 19836570; 1873-3573 eng

1535. Xiong, J. F.; Guan, Z.; Zhou, G. M.; Tang, X. Y.; Lv, Y. J., and Wang, H. L. Determination of chlorpyrifos in environmental water samples by dispersive liquid-liquid microextraction with solidification of a floating organic drop followed by gas chromatography with flame photometry detection. 2012; 4, (10): 3246-3250.

Rec #: 72319
Notes: Chemical of Concern: CPY
Abstract: Abstract: Dispersive liquid-liquid microextraction with solidification of a floating organic drop (DLLME-SFO) followed by gas chromatography with flame photometry detection (GC-FPD) has been adopted and improved for determining chlorpyrifos in environmental water samples. A mixture of 1.5 mL of methanol (disperser solvent) and 40 mu L of 1-dodecanol (extraction solvent) was quickly injected into 25 mL of a water sample with a liquid-transferring device. After five minutes, 0.5 g of sodium chloride was added into the aqueous solution and the sample vial was shaken by hand. Another five minutes later, the sample was centrifugated at 3400 rpm for 3 min, and then the centrifugal tube was placed in an ice bath. When the extraction solvent floating on the top of the aqueous solution had solidified, it was transferred into another vial and diluted with 60 mu L of ethyl acetate. The extraction recovery (ER) and the enrichment factor (EF) were 79.02% and 232.42, respectively. The limit of detection (LOD) (S/N = 3) was 0.02 mu g L(-1), the linear range was 0.05-4 mu g L(-1), the relative standard deviation (RSD) was in the range of 3.93-7.29% and the recoveries of spiked samples ranged from 84.34% to 110.20%.
ISI Document Delivery No.: 012LS

1536. Xiong, J. F.; Tang, X. Y.; Zhou, G. M.; Guan, Z., and Wu, L. M. Dispersive solid phase extraction coupled with HPLC-UV for simultaneous determination of chlorpyrifos and 3,5,6-trichloro-2-pyridinol in soil samples. 2013; 5, (2): 536-540.

Rec #: 72329
Keywords: FATE
Notes: Chemical of Concern: CPY
Abstract: Abstract: A simple method was proposed for simultaneous determination of chlorpyrifos and its hydrolysis and biodegradation product 3,5,6-trichloro-2-pyridinol (TCP) in soil samples. The analytes were extracted with ethyl acetate (EtOAc) under optimized pretreatment conditions such as the type, volume and pH of extraction solvent. Clean-up of the extract from soil was conducted by dispersive solid phase extraction (DSPE) using activated carbon as the adsorbent. High performance liquid chromatography with ultraviolet detection (HPLC-UV) was used for simultaneous determination of the two analytes. Under the optimized HPLC conditions, the limits of detection (LOD), obtained on signal-to-noise ratio (S/N) of 3, for chlorpyrifos and TCP were 0.0080 mg kg(-1) and 0.0120 mg kg(-1), respectively. Linear calibration curves were obtained in the range of 0.05-2.00 mg kg(-1) and 0.03-2.00 mg kg(-1) for chlorpyrifos and TCP, respectively. The spike recoveries from soil ranged from 89.4% to 114%, and the relative standard deviation (RSD) was in the range of 0.80%-14%.
ISI Document Delivery No.: 058TZ

1537. Xu, F; Chang, X; Lou, D; Wu, Q; Zhou, Z, and Xu, F. Chlorpyrifos Exposure Causes Alternation in Dopamine Metabolism in Pc12 Cells. 2012 May; 22, (4): 309-314.

Rec #: 38849
Notes: Chemical of Concern: CPY
Abstract: Abstract: Chlorpyrifos (CPF) is one of the organophosphorus pesticides widely used around the world, especially in China. Acetylcholinesterase inhibition is the main effect of organophosphorus insecticides exposure. Studies showed that CPF may also interfere with the metabolism of monoamine transmitters. To investigate the effects of CPF on dopaminergic pathway, the dopamine content, gene expression of catechol-O-methyl- transferase (COMT), vesicular monoamine transporter-2 (VMAT-2), and monoamine oxidase (MAO) and its activity in PC12 cells exposed to CPF was determined. Results showed that cell viability was decreased and total dopamine concentration was increased with CPF administration in a dose-dependent pattern. Gene of MAO was significantly downregulated in PC12 cells, while genes of COMT and VMAT-2 in PC12 cells did not show any change after CPF exposure. The MAO activity was decreased following incubation exposed to CPF. These results suggest that CPF may interfere with dopaminergic pathway through inhibition on gene and protein expression of MAO in vitro.
Keywords: Gene expression
Keywords: Chlorpyrifos
Keywords: Pesticides (organophosphorus)
Keywords: Insecticides
Keywords: Dopamine
Keywords: Pheochromocytoma cells
Keywords: Acetylcholinesterase
Keywords: Amine oxidase (flavin-containing)
Keywords: X 24330:Agrochemicals
Keywords: Toxicology Abstracts
Keywords: Metabolism
Date revised - 2012-10-01
Language of summary - English
Pages - 309-314
ProQuest ID - 1093475613
SubjectsTermNotLitGenreText - Gene expression; Chlorpyrifos; Pesticides (organophosphorus); Insecticides; Dopamine; Pheochromocytoma cells; Acetylcholinesterase; Amine oxidase (flavin-containing); Metabolism
Last updated - 2012-10-19
British nursing index edition - Toxicology Mechanisms and Methods [Toxicol. Mech. Methods]. Vol. 22, no. 4, pp. 309-314. May 2012.
Corporate institution author - Chang, X; Lou, D; Zhou, Z
DOI - MD-0019808024; 17172008; 1537-6516 English

1538. Xu, J. F.; Li, W. J.; Chen, X. Y., and Zhou, Y. Klebsiella alba sp nov., a novel pesticide-tolerant bacterium from a heavily polluted environment. 2010; 56, (3): 241-247.

Rec #: 72359
Keywords: BACTERIA
Notes: Chemical of Concern: CPY
Abstract: Abstract: A novel Gram-negative, aerobic, rod-shaped and pesticide (chlorpyrifos and atrazine) tolerant strain, designated CW-D 3(T), was isolated from a heavily polluted soil sample in Jiangsu Province, China. The strain could tolerate about 800 mu atrazine or chlorpyrifos when added in TYB medium. A polyphasic taxonomy including phylogenetic and phenotypic analysis was performed on the new isolate. Phylogenetic analysis and chemotaxonomic characteristics revealed that strain CW-D 3(T) belongs to the genus Klebsiella. Its closest phylogenetic neighbors were K. singaporensis (strain LX3(T)), three subspecies of K. pneumoniae (strains ATCC 13883(T), ATCC 13884(T) and ATCC 11296(T)) and K. granulomatis, according to 16S rRNA and rpoB gene analysis. The 16S rRNA gene sequence similarity was about 94.8-97.6% and the rpoB gene sequence similarity was about 96.8-98.6% to its phylogenetic neighbors. Strain CW-D 3(T) also showed low DNA-DNA reassociationalues (less than 21%) with respect to the three subspecies of K. pneumoniae and Klebsiella singaporensis, its major fatty acids were C(14:0), C(16:0), c-C(17:0) and c-C(19:0) omega 8c. The G+C content of the DNA was 58.3 mol%. Phylogenetic and phenotypic results supported the assignment of CW-D 3(T) as a novel species of the genus Klebsiella, for which the name Klebsiella alba sp. nov. is proposed. The type strain is CW-D 3(T) (=LMG 24441(T)=KCTC 12878(T) =CCTCC AB 206144(T)).
Keywords: Klebsiella alba, polluted environment, polyphasic taxonomy, rpoB gene,
ISI Document Delivery No.: 847CC

1539. Xu, W. N.; Liu, W. B., and Liu, Z. P. Trichlorfon-induced apoptosis in hepatocyte primary cultures of Carassius auratus gibelio. 2009; 77, (7): 895-901.

Rec #: 72379
Keywords: IN VITRO
Notes: Chemical of Concern: CPY
Abstract: Abstract:
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