Viability of human periodontal ligament cells in four different storage/transport media at five different time intervals.
Brief resume of the study intended work
Need for the study
Oral trauma resulting in tooth avulsion creates a special problem for the child and the Pediatric dentist.1 Avulsion is a complex injury that affects multiple tissues. It accounts for upto 16% of all traumatic injuries in the permanent dentition.2 The injuries occur mainly in the school going children in the age group of 7-10 years,accidents on the school playground being the most common cause.3,4 Usually the avulsed tooth kept in a dry condition shows poor prognosis. When immediate replantation of an avulsed tooth is not possible, the storage conditions should be designed to maximize preservation of the periodontal ligament cells during transportation to the dental office2. So a study is required to come out with an effective, economical and easily available storage/transport media for improving the prognosis of the replanted tooth.
1 to assess the viability of the periodontal ligament cells in four different storage media which are easily available at places with higher risks of accidents i.e. in school / house.
2. to assess the viability of the periodontal ligament cells at five different time intervals.
Review of the literature
1. Olson Brian D. , Mailhot Jason M. , Anderson Ronald W. , Schuster George S. , Weller R. Norman ( 1997)1
Conducted a study to determine the ability of various solutions to maintain human periodontal ligament cell viability in-vitro. Solutions used were Milk, Save-A-Tooth, Save-A-Tooth supplemented with platelet derived growth factor-BB (PDGF) and Gatorade.
This study concluded that Milk and Save-A-Tooth with PDGF were suitable as transport medium for avulsed teeth.
2. Ashkenazi M. , Sarnat H. , Keila S. ( 1999)3
Evaluated the effectiveness of six different media: Culture medium, α minimal essential medium, Milk, Hank’s balanced salt solution, Viaspan and Conditioned medium to preserve cultured periodontal ligament fibroblast.
In conclusion ,Hank’s balanced salt solution and Milk were the most effective media for preserving the viability, mitogenicity and clonogenic capacity after storage for upto 24hrs at 4 0C.
3. Fagade Olusegun O. ( 2005)5
Reviewed the literature to find an appropriate storage media to maintain or improve the viability of the cells during extra-alveolar period. Storage media proposed in the literature include Eagle’s culture media, Hank’s balanced salt solution (HBSS), Patients own serum, Isotonic saline, Tap water, Saliva and Pasteurized milk. The most favoured ones are Hank’s balanced salt solution (HBSS), Patients own serum, Eagle’s culture medium, Pasteurized milk and Isotonic saline.
Conducted a long term clinical study to investigate the healing capacity following avulsion and replantation. A tooth rescue box was developed and distributed to offer optimal storage conditions for avulsed tooth. The therapy comprised extraoral endodontic treatment and application of medicaments to enhance periodontal ligament healing. The authors recommended good prognosis for the avulsed teeth when stored immediately in a cell- compatible medium.
7.Materials and Methods
7.1 Source of data
The study sample consists of 60 premolars, extracted for orthodontic purpose reporting to the Department of Oral Surgery, SDM College of Dental Sciences. These teeth are immediately transferred to tissue culture medium and transported to the laboratory .
7.2 Method of collection of data
For the study the tooth is removed from the tissue culture media, rinsed thoroughly with Sterile Isotonic Saline to take out all the tissue culture residues.
Using No. 15 B.P. blade the periodontal ligament is scraped from the root surface and collected in the selected four different storage media. The viability of the periodontal ligament cells are checked under the Inverted Tissue Microscope after staining with 0.4% Tryphan blue at 5 different time intervals i.e. 1,2 4,6 and 24 hours.
Does your study require any investigation to be conducted on humans or animals.
Has Ethical clearance been obtained from your institution.
List of references
Olson Brian D. , Mailhot Jason M. , Anderson Ronald W. , Schuster George S. , Weller R. Norman. 1997 “Comparison of various transport media on human periodontal ligament cell viability”. Journal of Endodontics, 23:76-679.
Sigalas Emmanouil , Regan John D. , Kramer Phillip R. , Witherspoon David E. , Opperman Lynne A. 2004 “ Survival of human periodontal ligament cells in media proposed for transport of avulsed teeth”. Dental Traumatology, 20:21-28.
Ashkenazi M. , Sarnat H. , Keila S. 1999 “In vitro viability, mitogenicity and clonogenic capacity of periodontal ligament cells after storage in six different media”. Endodontics and Dental Traumatology, 15:149-156.
Andreasen J.O. , Traumatic injuries of the teeth. Copenhagen: Munksgaard, 1981.25pp.
Fagade Olusegun O. 2005 “Extra-Alveolar storage
media for tooth autotransplants and replants”. The
Pohl Yango, Filippi Andreas, Kirschner H. 2005 “Results after replantation of avulsed permanent teeth. II. Periodontal healing and the role of physiologic storage and antiresorptive-regenerative therapy” Dental Traumatology, 21:93-101.